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Characterization of Bacterial Symbionts in Deep-Sea Fauna: Protocols for Sample Conditioning, Fluorescence In Situ Hybridization, and Image Analysis

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Hydrocarbon and Lipid Microbiology Protocols

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Symbioses with bacteria are key adaptations allowing various groups of metazoans to reach high biomasses at deep sea reducing habitats including hydrothermal vents and cold seeps. Characterizing these associations is challenging due to the constraints associated with work on deep-sea organisms. These include limited sample availability, impact of recovery procedures and shipment on sample quality, and general lack of environmental data. In this chapter, a standard procedure for sample processing at sea which can maximize sample use back in the laboratory is presented, with example protocols for sample fixation, fluorescence in situ hybridization (FISH)-based localization of symbionts in animal tissues, and estimation of their relative abundances in the case of multiple symbioses.

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Authors and Affiliations

  1. Sorbonne Universités, UPMC Université Paris 06, UMR 7208 BOREA, Adaptation aux Milieux Extrêmes, 75005, Paris, France

    Sébastien Duperron

  2. Institut Universitaire de France, Paris, France

    Sébastien Duperron

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  1. Sébastien Duperron
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Correspondence to Sébastien Duperron .

Editor information

Editors and Affiliations

  1. University of Essex, School of Biological Sciences University of Essex, Colchester, Essex, United Kingdom

    Terry J. McGenity

  2. Institute of Microbiology, Technical University Braunschweig, Braunschweig, Germany

    Kenneth N. Timmis

  3. Department of Biology, University of the Balearic Islands and Mediterranean Institute for Advanced Studies (IMEDEA, UIB-CSIC), Palma de Mallorca, Spain

    Balbina Nogales

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Duperron, S. (2015). Characterization of Bacterial Symbionts in Deep-Sea Fauna: Protocols for Sample Conditioning, Fluorescence In Situ Hybridization, and Image Analysis. In: McGenity, T., Timmis, K., Nogales , B. (eds) Hydrocarbon and Lipid Microbiology Protocols. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/8623_2015_73

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  • DOI: https://doi.org/10.1007/8623_2015_73

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-53116-7

  • Online ISBN: 978-3-662-53118-1

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