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Kupffer Cell Protein Isolation and Detection by Western Blot

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Kupffer Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2164))

Abstract

Kupffer cells are the majority of tissue macrophages present in the body and are actively involved in the regulation of liver homeostasis as well as in the progression of liver diseases. Here we describe a methodology to successfully isolate and detect proteins from Kupffer cells and avoid the contamination of other liver cell populations.

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Correspondence to Valerio Azzimato .

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Azzimato, V. (2020). Kupffer Cell Protein Isolation and Detection by Western Blot. In: Aouadi, M., Azzimato, V. (eds) Kupffer Cells. Methods in Molecular Biology, vol 2164. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0704-6_4

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  • DOI: https://doi.org/10.1007/978-1-0716-0704-6_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0703-9

  • Online ISBN: 978-1-0716-0704-6

  • eBook Packages: Springer Protocols

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