Abstract
In Pseudomonas aeruginosa, identification of new partners of a protein of interest could give precious clues to decipher a biological process in which this protein is involved. However, genes encoding for partners of a protein of interest are unknown and frequently scattered throughout the genome. We describe herein the construction and the use of pan-genomic bacterial two-hybrid libraries to identify new partners of a protein of interest encoded by P. aeruginosa.
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Acknowledgements
The work of C. B. and S. de B. is supported by the French Cystic Fibrosis Foundation (VLM), and CNRS institutional and ANR grants: ERA-NET ADHRES 27481, PCV-ANR 27628, ANR Jeunes Chercheurs JC09-473544 and GDR3171, as well as Europathogenomics 2005–2010 REX LSHB-CT-2005512061-EPG.
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de Bentzmann, S., Bordi, C. (2014). Construction of Pseudomonas aeruginosa Two-Hybrid Libraries for High-Throughput Assays. In: Filloux, A., Ramos, JL. (eds) Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol 1149. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-0473-0_19
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DOI: https://doi.org/10.1007/978-1-4939-0473-0_19
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