Abstract
Although the completion and annotation of the entire genomic DNA sequence of Pseudomonas aeruginosa PAO1 strain has been carried out, an important number of genes are still of unknown function and many genetic elements involved in various regulatory pathways like small RNA are still unrevealed. One of the strategies to identify gene function or genetic elements is the construction and utilization of DNA genomic library. Here, we describe the construction a P. aeruginosa DNA genomic library.
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Acknowledgements
The work of C.B. has been supported by the French Cystic Fibrosis Foundation (VLM), and CNRS institutional and ANR grant: Two-CompNet (2005–2009), as well as Europathogenomics 2005–2010 REX LSHB-CT-2005512061-EPG.
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Bordi, C. (2014). Construction of a Pseudomonas aeruginosa Genomic DNA Library. In: Filloux, A., Ramos, JL. (eds) Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol 1149. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-0473-0_42
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DOI: https://doi.org/10.1007/978-1-4939-0473-0_42
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Publisher Name: Humana, New York, NY
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