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Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-arming Technology

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Yeast Metabolic Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1152))

Abstract

The cell surface display system in yeast enables the innovative strategy for improving cellular functions in a wide range of applications such as biofuel production, bioremediation, synthesis of valuable chemicals, recovery of rare metal ions, development of biosensors, and high-throughput screening of proteins/peptides library. Display of enzymes for polysaccharide degradation enables the construction of metabolically engineered whole-cell biocatalyst owing to the accessibility of the displayed enzymes to high-molecular-weight polysaccharides. In addition, along with fluorescence-based activity evaluation, fluorescence-activated cell sorting (FACS), and yeast cell chip, the cell surface display system is an effective molecular tool for high-throughput screening of mutated proteins/peptides library. In this article, we describe the methods for cell surface display of proteins/peptides of interest on yeast, evaluation of display efficiency, and harvesting of the displayed proteins/peptides from cell surface.

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Authors and Affiliations

  1. Laboratory of Biomacromolecular Chemistry, Division of Applied Life Sciences, Department of Applied Biochemistry, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan

    Kouichi Kuroda & Mitsuyoshi Ueda

Authors
  1. Kouichi Kuroda
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  2. Mitsuyoshi Ueda
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Correspondence to Mitsuyoshi Ueda .

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Editors and Affiliations

  1. Industrial Biotechnology, Chalmers University of Technology, Gothenburg, Sweden

    Valeria Mapelli

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Kuroda, K., Ueda, M. (2014). Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-arming Technology. In: Mapelli, V. (eds) Yeast Metabolic Engineering. Methods in Molecular Biology, vol 1152. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0563-8_8

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  • DOI: https://doi.org/10.1007/978-1-4939-0563-8_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0562-1

  • Online ISBN: 978-1-4939-0563-8

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