Abstract
Chemotaxis assays have a number of applications in the study of leukocyte biology and immune/inflammatory pathology. Multiwell “blind chamber”-type assays allow a large number of parallel measurements within a single assay. The development of fluorescence assays using microplate-based chemotaxis chambers has permitted a degree of automation to be applied to these assays. Here, a method is described for the quantitative measurement of eosinophil migration using a 96-well assay with numbers of cells that may realistically be obtained from blood samples.
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References
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Dent, G. (2014). Eosinophil Chemotaxis. In: Walsh, G. (eds) Eosinophils. Methods in Molecular Biology, vol 1178. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1016-8_10
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DOI: https://doi.org/10.1007/978-1-4939-1016-8_10
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1015-1
Online ISBN: 978-1-4939-1016-8
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