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Use of Cocultures for the Study of Cellular Interactions Influencing B Cell Regulatory Functions

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Regulatory B Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1190))

Abstract

Many immunological processes are contextually controlled by complex interactions among different cell types. Several studies have shown that B cells produce the immune regulatory cytokine IL-10 in response to different external stimuli but also to immune-mediated signals. Endogenous signals that derive from the cross talk between B lymphocytes and other cells of the immune system can affect IL-10 production by B cells in both physiological and pathological conditions. With the aim to provide a guide for the study of how partner cells can induce IL-10-producing B cells, here we describe the protocols to investigate IL-10 production at a single cell level in a dendritic cell-B cell coculture in vitro system. These methods are a proof of concept that can be easily extrapolated and adapted to the study of the interaction between B cells and other immune cell types.

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Authors and Affiliations

  1. Department of Medical and Biological Sciences, University of Udine, P.le M. Kolbe 4, 33100, Udine, Italy

    Francesca Mion, Carlo Pucillo & Gaetano Vitale

  2. International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34149, Trieste, Italy

    Federica Benvenuti

Authors
  1. Francesca Mion
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  2. Federica Benvenuti
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  3. Carlo Pucillo
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  4. Gaetano Vitale
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Corresponding author

Correspondence to Francesca Mion .

Editor information

Editors and Affiliations

  1. Department of Medical and Biological Sciences, University of Udine, Udine, Italy

    Gaetano Vitale

  2. Department of Medical and Biological Sciences, University of Udine, Udine, Italy

    Francesca Mion

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Mion, F., Benvenuti, F., Pucillo, C., Vitale, G. (2014). Use of Cocultures for the Study of Cellular Interactions Influencing B Cell Regulatory Functions. In: Vitale, G., Mion, F. (eds) Regulatory B Cells. Methods in Molecular Biology, vol 1190. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1161-5_12

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  • DOI: https://doi.org/10.1007/978-1-4939-1161-5_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1160-8

  • Online ISBN: 978-1-4939-1161-5

  • eBook Packages: Springer Protocols

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