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Identification of (Antioxidative) Plants in Herbal Pharmaceutical Preparations and Dietary Supplements

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Advanced Protocols in Oxidative Stress III

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1208))

Abstract

The standard procedures for the identification, authentication, and quality control of medicinal plants and herbs are nowadays limited to pure herbal products. No guidelines or procedures, describing the detection or identification of a targeted plant or herb in pharmaceutical preparations or dietary supplements, can be found. In these products the targeted plant is often present together with other components of herbal or synthetic origin.

This chapter describes a strategy for the fast development of a chromatographic fingerprint approach that allows the identification of a targeted plant in herbal preparations and dietary supplements. The strategy consists of a standard chromatographic gradient that is tested for the targeted plant with different extraction solvents and different mobile phases. From the results obtained, the optimal fingerprint is selected. Subsequently the samples are analyzed according to the selected methodological parameters, and the obtained fingerprints can be compared with the one obtained for the pure herbal product or a standard preparation. Calculation of the dissimilarity between these fingerprints will result in a probability of presence of the targeted plant. Optionally mass spectrometry can be used to improve specificity, to confirm identification, or to identify molecules with a potential medicinal or antioxidant activity.

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References

  1. Aspöck H, Aver H, Picher O (1996) Trichuris trichiura eggs in the neolithic glacier mummy from the Alps. Parasitol Today 12:255–256

    Article  Google Scholar 

  2. Fujii Y, Fujii H, Yamazaki M (1983) Separation and determination of cardiac glycosides in digitalis purpurea leaves by micro high-performance liquid chromatography. J Chromatogr 258:147–153

    Article  CAS  Google Scholar 

  3. Miller RJ, Jolles C, Rapoport H (1973) Morphine metabolism and normorphine in Papaver somniferum. Phytochemistry 12: 597–603

    Article  CAS  Google Scholar 

  4. Strobel G, Stierle A, Hess WM (1994) The stimulation of taxol production in Taxus brevifolia by various growth retardants. Plant Sci 101:115–124

    Article  CAS  Google Scholar 

  5. Phillipson JD, Handa SS (1976) Hyoscyamine N-oxide in Atropa belladonna. Phytochemistry 15:605–608

    Article  CAS  Google Scholar 

  6. Datta A, Srivastava PS (1997) Variation in vinblastine production by Catharanthus roseus during in vivo and in vitro differentiation. Phytochemistry 46:135–137

    Article  CAS  Google Scholar 

  7. World Health Organization (2002) Traditional Medicine Strategy 2002–2005, Geneva, Switzerland. http://whqlibdoc.who.int/hq/2002/who_edm_trm_2002.1.pdf. Accessed 7 May 2013

  8. World Health Organization (1991) Guidelines for the assessment of herbal medicines, Geneva, Switzerland. http://whqlibdoc.who.int/HQ/1991/WHOTRM91.4.pdf. Accessed 7 May 2013

  9. World Health Organization (2005) National policy on traditional medicine and regulation of herbal medicines, Geneva, Switzerland. http://whqlibdoc.who.int/publications/2005/9241593237.pdf. Accessed 7 May 2013

  10. World Health Organization (2000) General guidelines for methodologies on research and evaluation of traditional medicine, Geneva, Switzerland. http://whqlibdoc.who.int/hq/2000/WHOEDMTRM2000.1.pdf. Accessed 7 May 2013

  11. European Medicine Evaluation Agency (2010) Inventory of herbal substances for assessment–Alphabetical order, London, UK. http://www.ema.europa.eu/docs/enGB/document library/Other/2009/12/WC500017723.pdf. Accessed 7 May 2013

  12. European Medicine Evaluation Agency (2010) Guideline on declaration of herbal substances and herbal preparations in herbal medicinal products/traditional herbal medicinal products, London, UK. http://www.ema.europa.eu/docs/enGB/document library/Scientific guideline/2009/09/WC500003283.pdf. Accessed 7 May 2013

  13. European Medicine Evaluation Agency (2006) Guideline on the environmental risk assessment of medicinal products for human use, London, UK. http://www.ema.europa.eu/docs/enGB/document library/Scientificguideline/2009/10/WC500003978.pdf. Accessed 7 May 2013

  14. Liang Q, Qu J, Luo G et al (2006) Rapid and reliable determination of illegal adulterant in herbal medicines and dietary supplements by LC/MS/MS. J Pharm Biomed Anal 40:305–311

    Article  PubMed  CAS  Google Scholar 

  15. Cianchino V, Acosta G, Ortega C et al (2008) Analysis of potential adulteration in herbal medicines and dietary supplements for the weight control by capillary electrophoresis. Food Chem 108:1075–1081

    Article  CAS  Google Scholar 

  16. Lopez-Feria S, Cardenas S, Garcıa-Mesa JA et al (2008) Classification of extra virgin olive oils according to the protected designation of origin, olive variety and geographical origin. Talanta 75:937–943

    Article  PubMed  CAS  Google Scholar 

  17. Bashir HA, Abu-Goukh ABA (2003) Compositional changes during guava fruit ripening. Food Chem 80:557–563

    Article  CAS  Google Scholar 

  18. Shin Y, Liu RH, Nock JF et al (2007) Temperature and relative humidity effects on quality, total ascorbic acid, phenolics and flavonoid concentrations, and antioxidant activity of strawberry. Postharv Biol Technol 45:349–357

    Article  CAS  Google Scholar 

  19. Tan X, Li Q, Chen X, Wang Z et al (2008) Simultaneous determination of 13 bioactive compounds in Herba Artemisiae Scopariae (Yin Chen) from different harvest seasons by HPLC-DAD. J Pharm Biomed Anal 47:847–853

    Article  PubMed  CAS  Google Scholar 

  20. Chena H, Li X, Chen J et al (2010) Simultaneous determination of eleven bioactive compounds in Saururus chinensis from different harvesting seasons by HPLC-DAD. J Pharm Biomed Anal 51:1142–1146

    Article  Google Scholar 

  21. Whitton PA, Lau A, Salisbury A et al (2003) Kava lactones and the kava-kava controversy. Phytochemistry 64:673–679

    Article  PubMed  CAS  Google Scholar 

  22. Simeoni P, Lebot V (2002) Identification of factors determining kavalactone content and chemotype in Kava (Piper methysticum Forst. f.). Biochem Syst Ecol 30:413–424

    Article  CAS  Google Scholar 

  23. Naithani V, Nair S, Kakkar P (2006) Decline in antioxidant capacity of Indian herbal teas during storage and its relation to phenolic content. Food Res Int 39:176–181

    Article  CAS  Google Scholar 

  24. Dufresne CJ, Farnworth ER (2001) A review of latest research findings on the health promotion properties of tea. J Nutr Biochem 12:404–421

    Article  PubMed  CAS  Google Scholar 

  25. Pastore RL, Fratellone P (2006) Potential health benefits of green tea (Camellia sinensis): a narrative review. Explore Diet Nutr 2:531–539

    Google Scholar 

  26. Wang H, Provan GJ, Helliwel K (2000) Tea flavonoids: their functions, utilization and analysis. Trends Food Sci Technol 11(2000):152–160

    Article  CAS  Google Scholar 

  27. Khan N, Mukhtar H (2007) Tea polyphenols for health promotion. Life Sci 81(2007):519–533

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  28. Re R, Pellegrini N, Proteggente A et al (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231–1237

    Article  PubMed  CAS  Google Scholar 

  29. Miller NJ, Rice-Evans CA (1996) Spectrophotometric determination of antioxidant activity. Redox Rep 2:161–171

    CAS  Google Scholar 

  30. Koleva I, van Beek T, Linssen J et al (2002) Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem Anal 13:8–17

    Article  PubMed  CAS  Google Scholar 

  31. Dai J, Mumper RJ (2010) Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 15:7313–7352

    Article  PubMed  CAS  Google Scholar 

  32. Paiva SAR, Russel RM (1999) β-Carotene and other carotenoids as antioxidants. J Am Coll Nutr 18:426–433

    Article  PubMed  CAS  Google Scholar 

  33. Yang CS, Lambert JD, Sang S (2009) Antioxidative and anti-carcinogenic activities of tea polyphenols. Arch Toxicol 83:11–21

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  34. Declaration of Alma-Ata (1978) International Conference on Primary Health Care, Alma-Ata, USSR. http://www.who.int/hpr/NPH/docs/declarationalmaata.pdf. Accessed 7 May 2013

  35. World Health Organization (1999) WHO monographs on selected medicinal plants (Volume I), Geneva, Switzerland. http://whqlibdoc.ho.int/publications/1999/9241545178.pdf. Accessed 7 May 2013

  36. World Health Organization (2003) WHO monographs on selected medicinal plants (Volume II),, Geneva, Switzerland. http://whqlibdoc.who.int/publications/2002/9241545372.pdf. Accessed 7 May 2013

  37. World Health Organization (2007) WHO monographs on selected medicinal plants (Volume III),, Geneva, Switzerland. http://apps.who.int/medicinedocs/index/assoc/s14213e/s14213e.pdf. Accessed 7 May 2013

  38. Committee on Herbal Medicinal Products (HMPC) (2010) Overview of assessment work – priority list, European Medicine Evaluation Agency, London, UK. http://www.ema.europa.eu/docs/enGB/document library/Other/2009/12/WC500017724.pdf. Accessed 7 May 2013

  39. European Pharmacopoeia 7.0 (2010) Council of Europe. Strasbourg, France

    Google Scholar 

  40. World Health Organization (WHO) (1999) Counterfeit drugs—guidelines for the development of measures to combat counterfeit drugs. WHO/EDM/QSM/99.1. Geneva, Switzerland

    Google Scholar 

  41. Newton P, Green M, Fernande`z F et al (2006) Counterfeit anti-infective drugs. Lancet Infect Dis 6:602–613

    Article  PubMed  CAS  Google Scholar 

  42. WHO (2009) Sixty-second World Health Assembly Item 12.9: counterfeit medical products. http://aps.who.int/gb/ebhwa/pdf_files/A62/A62_13-en.pdf. Accessed 7 May 2013

  43. Savaliya AA, Shah RP, Prasad B et al (2010) Screening of Indian aphrodisiac ayurvedic/herbal healthcare products for adulteration with sildenafil, tadalafil and/or vardenafil using LC/PDA and extracted ion LC-MS/TOF. J Pharm Biomed Anal 52:406–409

    Article  PubMed  CAS  Google Scholar 

  44. Kim SH, Lee J, Yoon T et al (2009) Simultaneous determination of anti-diabetes/anti-obesity drugs by LC/PDA, and targeted analysis of sibutramine analog in dietary supplement by LC/MS/MS. Biomed Chrom 23:1259–1265

    Article  CAS  Google Scholar 

  45. Stypulkowska K, Blazewicz A, Maurin J et al (2011) X-ray powder diffractometry and liquid chromatography studies of sibutramine and its analogues content in herbal dietary supplements. J Pharm Biomed Anal 56: 969–975

    Article  PubMed  CAS  Google Scholar 

  46. Tang MH, Chen SP, Ng SW et al (2011) Case series on a diversity of illicit weight-reducing agents: from the well-known to the unexpected. Br J Clin Pharmacol 71:250–253

    Article  PubMed  PubMed Central  Google Scholar 

  47. Venhuis BJ, Vredenbregt MV, Kaun N et al (2011) The identification of rimonabant polymorphs, sibutramine and analogues of both in counterfeit Acomplia bought on the internet. J Pharm Biomed Anal 54:21–26

    Article  PubMed  CAS  Google Scholar 

  48. Wang J, Chen B, Yao S (2008) Analysis of six synthetic adulterants in herbal weight-reducing dietary supplements by LC electrospray ionization-MS. Food Addit Contam Part A 25:822–830

    Article  CAS  Google Scholar 

  49. Tistaert C, Dejaegher B, Vander Heyden Y (2011) Chromatographic separation techniques and data handling methods for herbal fingerprints: a review. Anal Chim Acta 690:148–161

    Article  PubMed  CAS  Google Scholar 

  50. Massart DL, Vandeginste BGM, Buydens LMC et al (1997) Handbook of chemometrics and qualimetrics-part A. Elsevier Science, Amsterdam

    Google Scholar 

  51. Dumarey M, Smets I, Vander Heyden Y (2010) Prediction and interpretation of the antioxidant capacity of green tea from dissimilar chromatographic fingerprints. J Chromatogr B Analyt Technol Biomed Life Sci 878:2733–2740

    Article  PubMed  CAS  Google Scholar 

  52. Deconinck E, De Leersnijder C, Custers D et al (2013) A strategy for the identification of plants in illegal pharmaceutical preparations and food supplements using chromatographic fingerprints. Anal Bioanal Chem 405:2341–2352

    Article  PubMed  CAS  Google Scholar 

  53. Van Hong NT, Rivière C, Hong QT et al (2011) Identification by LC-ESI-MS of flavonoids responsible for the antioxidant properties of Mallotus species from Vietnam. Nat Prod Commun 6:813–818

    PubMed  CAS  Google Scholar 

  54. Tistaert C, Dejaegher B, Chataigné G et al (2012) Potential antioxidant compounds in Mallotus species fingerprints. Part II: fingerprint alignment, data analysis and peak identification. Anal Chim Acta 721:35–43

    Article  PubMed  CAS  Google Scholar 

  55. Thiangthum S, Dejaegher B, Goodarzi M et al (2012) Potentially antioxidant compounds indicated from Mallotus and Phyllanthus species fingerprints. J Chromatogr B Analyt Technol Biomed Life Sci 910:114–121

    Article  PubMed  CAS  Google Scholar 

  56. Deconinck E, Sacré PY, Courselle P (2013) Chromatography in the detection and characterization of illegal pharmaceutical preparations. J Chromatogr Sci. doi:10.1093/chromsci/bmt006

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Division of Food, Medicines and Consumer Safety, Section Medicinal Products, Scientific Institute of Public Health (WIV-ISP), Rue Juliette Wytsmanstraat 14, Brussels, 1050, Belgium

    Eric Deconinck, Deborah Custers & Jacques Omer De Beer

Authors
  1. Eric Deconinck
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  2. Deborah Custers
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  3. Jacques Omer De Beer
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Corresponding author

Correspondence to Eric Deconinck .

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Editors and Affiliations

  1. University of Florida, Gainsville, Florida, USA

    Donald Armstrong

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Deconinck, E., Custers, D., De Beer, J.O. (2015). Identification of (Antioxidative) Plants in Herbal Pharmaceutical Preparations and Dietary Supplements. In: Armstrong, D. (eds) Advanced Protocols in Oxidative Stress III. Methods in Molecular Biology, vol 1208. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1441-8_14

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  • DOI: https://doi.org/10.1007/978-1-4939-1441-8_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1440-1

  • Online ISBN: 978-1-4939-1441-8

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