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Identification of microRNAs in Human Plasma

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Osteoporosis and Osteoarthritis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1226))

Abstract

In recent years, microRNAs (miRNA) have been demonstrated to be present in body fluids and may therefore serve as diagnostic markers for diseases. By characterizing miRNA profiles in plasma, a miRNA signature may potentially be developed as a diagnostic and risk assessment tool for particular (patho)physiological states. This chapter describes the isolation, purification, identification, and sequencing of human plasma miRNAs.

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Acknowledgment

We thank Tanja Strini for excellent technical assistance. This work was supported by the Netherlands Organization for Scientific Research (NWO; NGI Horizon grant 93511016).

Author information

Authors and Affiliations

  1. Department of Internal Medicine, Erasmus MC, Room Ee585b, 3000 DR, 1738, Rotterdam, The Netherlands

    Bram C. van der Eerden Ph.D., Rodrigo D. Alves, Marijke Schreuders-Koedam, Jeroen van de Peppel & Johannes P. van Leeuwen

  2. Erasmus Center for Biomics, Erasmus MC, Rotterdam, The Netherlands

    Christel E. Kockx, Zeliha Ozgur & Wilfred F. van Ijcken

Authors
  1. Bram C. van der Eerden Ph.D.
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  2. Rodrigo D. Alves
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  3. Christel E. Kockx
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  4. Zeliha Ozgur
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  5. Marijke Schreuders-Koedam
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  6. Jeroen van de Peppel
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  7. Wilfred F. van Ijcken
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  8. Johannes P. van Leeuwen
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Corresponding author

Correspondence to Bram C. van der Eerden Ph.D. .

Editor information

Editors and Affiliations

  1. Mayo Clinic, Rochester, Minnesota, USA

    Jennifer J. Westendorf

  2. Mayo Clinic, Rochester, Minnesota, USA

    Andre J. van Wijnen

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© 2015 Springer Science+Business Media. New York

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van der Eerden, B.C. et al. (2015). Identification of microRNAs in Human Plasma. In: Westendorf, J., van Wijnen, A. (eds) Osteoporosis and Osteoarthritis. Methods in Molecular Biology, vol 1226. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1619-1_7

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  • DOI: https://doi.org/10.1007/978-1-4939-1619-1_7

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1618-4

  • Online ISBN: 978-1-4939-1619-1

  • eBook Packages: Springer Protocols

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