Abstract
The domains of large ribozymes are composed of secondary structural elements that fold independently in solution. Interactions between these structures contribute to the overall structure of the ribozyme. These elements can also be deleted, reducing catalytic activity, and their structure–function relationships determined by assaying the ability of variant elements to activate the deleted ribozyme in trans. This is illustrated for an unusual loop structure from Bacillus subtilis Ribonuclease P RNA.
An erratum to this chapter is available at http://dx.doi.org/10.1007/978-1-4939-1896-6_15
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-1896-6_15
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Acknowledgement
This work was supported by the NASA Grant NNX07AJ21G and the University of Missouri Research Board.
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Martin, M.B., Leeper, T.L., Schmidt, F.J. (2015). Transactivation of Large Ribozymes. In: Schmidt, F. (eds) RNA-RNA Interactions. Methods in Molecular Biology, vol 1240. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1896-6_5
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DOI: https://doi.org/10.1007/978-1-4939-1896-6_5
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