Abstract
A highly sensitive SPF10 real-time PCR was developed to achieve simultaneous amplification and detection of the human papillomavirus (HPV) target. That way, LiPA analysis of the HPV-negative samples can be avoided, reducing workload and cost. Here, we describe in detail a SYBR Green I-based real-time PCR assay based on SPF10 primers using the LightCycler® 480 system to generate and detect HPV amplicons, which are compatible with the LiPA assay.
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Acknowledgement
This work was financially supported by the Industrial Research Fund of the University of Antwerp (IOF/SBO 3501/3494) and Innogenetics NV. We would like to thank D. De Rijck for his assistance with the figures.
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Micalessi, M.I., Boulet, G.A., Bogers, J. (2015). A Real-Time PCR Approach Based on SPF10 Primers and the INNO-LiPA HPV Genotyping Extra Assay for the Detection and Typing of Human Papillomavirus. In: Keppler, D., Lin, A. (eds) Cervical Cancer. Methods in Molecular Biology, vol 1249. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2013-6_2
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DOI: https://doi.org/10.1007/978-1-4939-2013-6_2
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