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Targeted Re-sequencing in Psychiatric Disorders

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Applied Neurogenomics

Part of the book series: Neuromethods ((NM,volume 97))

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Abstract

The method presented here enables researchers to sequence a target region in the genome in a large number of subjects at comparatively low cost. The method relies heavily on modern massively parallel sequencing (MPS) technologies, and the costs and workload for DNA sample preparation are major factors in targeted re-sequencing studies involving many individual samples. In order to reduce costs during sample preparation, we propose to sequence pools of subjects rather than individual subjects. Thus, our approach is divided into four subparts: (1) non-barcoded sample pooling, (2) enrichment of the target region, (3) sequencing using standard MPS protocols, and (4) tailored bioinformatics analysis of the data, which identifies rare variants in the pools.

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Author information

Authors and Affiliations

  1. Functional Imaging in Neurodegenerative Disorders Lab, Department of Neurology & Neurological Sciences, Stanford University, 780 Welch Road, CJ350 C38, Stanford, CA, 94304, USA

    Andre Altmann

  2. Department of Translational Research in Psychiatry, Max Planck Institute of Psychiatry, Munich, Germany

    Peter Weber

Authors
  1. Andre Altmann
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  2. Peter Weber
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Corresponding author

Correspondence to Andre Altmann .

Editor information

Editors and Affiliations

  1. Jain PharmaBiotech, Basel, Switzerland

    Kewal K. Jain

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Altmann, A., Weber, P. (2015). Targeted Re-sequencing in Psychiatric Disorders. In: Jain, K. (eds) Applied Neurogenomics. Neuromethods, vol 97. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2247-5_5

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  • DOI: https://doi.org/10.1007/978-1-4939-2247-5_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2246-8

  • Online ISBN: 978-1-4939-2247-5

  • eBook Packages: Springer Protocols

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