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Ca2+ Mobilization Assays in GPCR Drug Discovery

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G Protein-Coupled Receptor Screening Assays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1272))

Abstract

Intracellular calcium mobilization can be measured using several methods varying in indicator dyes and devices used. In this chapter, we describe the fluorescence-based method (FLIPR Calcium 4 Assay) developed by Molecular Devices for a FlexStation and routinely used in our laboratory for detecting intracellular calcium changes. The assay is designed to study calcium mobilization induced by majority of GPCRs and calcium channels and allows for simultaneous concentration-dependent analysis of several receptor agonists and antagonists, useful in receptor characterization and drug discovery projects.

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Authors and Affiliations

  1. Division of Asthma, Allergy and Lung Biology, King’s College London, 5th Floor, Tower Wing, Guy’s Hospital, London, SE1 9RT, UK

    Grzegorz Woszczek & Elisabeth Fuerst

  2. MRC & Asthma UK Centre in Allergic Mechanisms of Asthma, London, UK

    Grzegorz Woszczek & Elisabeth Fuerst

Authors
  1. Grzegorz Woszczek
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  2. Elisabeth Fuerst
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Corresponding author

Correspondence to Grzegorz Woszczek .

Editor information

Editors and Affiliations

  1. IBB – Institute for Biotechnology and Bioengineering, Lisbon, Portugal

    Duarte Miguel F. Prazeres  & Sofia Aires M. Martins  & 

  2. Department of Bioengineering, Instituto Superior Técnico Universidade de Lisboa, Lisbon, Portugal

    Duarte Miguel F. Prazeres

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Woszczek, G., Fuerst, E. (2015). Ca2+ Mobilization Assays in GPCR Drug Discovery. In: Prazeres, D.M.F., Martins, S.A.M. (eds) G Protein-Coupled Receptor Screening Assays. Methods in Molecular Biology, vol 1272. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-2336-6_6

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  • DOI: https://doi.org/10.1007/978-1-4939-2336-6_6

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-2335-9

  • Online ISBN: 978-1-4939-2336-6

  • eBook Packages: Springer Protocols

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