Abstract
Time-lapse atomic force microscopy (AFM) is widely used for direct visualization of the nanoscale dynamics of various biological systems. The advent of high-speed AFM instrumentation made it possible to image the dynamics of proteins and protein-DNA complexes within millisecond time range. This chapter describes protocols for studies of structure and dynamics of nucleosomes with time-lapse AFM including the high-speed AFM instrument. The necessary specifics for the preparation of chromatin samples for imaging with AFM including the protocols for the surface preparation are provided.
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Acknowledgements
The authors thank the members of the Lyubchenko lab and specifically N. Filenko and M. Atsushi for their contribution to the protocols on handling of nucleosomes for AFM imaging. The work is supported by grants from NIH (P01 GM091743, 1R01 GM096039) and NSF (EPS—1004094).
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Lyubchenko, Y.L., Shlyakhtenko, L.S. (2015). Chromatin Imaging with Time-Lapse Atomic Force Microscopy. In: Chellappan, S. (eds) Chromatin Protocols. Methods in Molecular Biology, vol 1288. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2474-5_3
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DOI: https://doi.org/10.1007/978-1-4939-2474-5_3
Publisher Name: Humana Press, New York, NY
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