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Measurement of Antibody-Mediated Reduction of Plasmodium yoelii Liver Burden by Bioluminescent Imaging

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Malaria Vaccines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1325))

Abstract

Antibodies against the infectious sporozoite stage of malaria have been shown to be effective in preventing infection of the liver and in mitigating the ensuing blood stage. However, only a handful of antibody targets have been vetted and shown to be successful in mediating in vivo protection. Even more limited are the means with which to measure how effectively antibodies can reduce the number of parasites establishing infection in the liver. Traditionally, only qPCR of infected mouse livers could accurately measure liver parasite burden. However, this procedure requires sacrifice of the animal and precludes monitoring of the ensuing blood stage infection. Herein we describe a method of accurately assessing antibody-mediated reduction of parasite liver burden by combining passive or active immunization of mice and mosquito bite challenge with luciferase-expressing transgenic P. yoelii parasites. This method is rapid, reliable and allows for observation of blood stage disease in the same animal. This model will prove integral in screening the efficacy of novel antibody targets as the search for a more effective malaria vaccine continues.

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Acknowledgements

We would like to thank the Center for Infectious Disease Research Center for Mosquito Production and Malaria Infection Research for their production of quality infected mosquitoes and parasites.

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Authors and Affiliations

  1. Center for Infectious Disease Research, 307 Westlake Avenue North, Suite 500, Seattle, WA, 98109, USA

    Brandon K. Sack, Jessica L. Miller, Ashley M. Vaughan & Stefan H. I. Kappe

Authors
  1. Brandon K. Sack
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  2. Jessica L. Miller
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  3. Ashley M. Vaughan
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  4. Stefan H. I. Kappe
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Correspondence to Brandon K. Sack .

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Editors and Affiliations

  1. Center for Infectious Disease Research, Seattle, Washington, USA

    Ashley Vaughan

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Sack, B.K., Miller, J.L., Vaughan, A.M., Kappe, S.H.I. (2015). Measurement of Antibody-Mediated Reduction of Plasmodium yoelii Liver Burden by Bioluminescent Imaging. In: Vaughan, A. (eds) Malaria Vaccines. Methods in Molecular Biology, vol 1325. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2815-6_6

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  • DOI: https://doi.org/10.1007/978-1-4939-2815-6_6

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2814-9

  • Online ISBN: 978-1-4939-2815-6

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