Abstract
Microfluidic devices offer several advantages for C. elegans research, particularly for presenting precise physical and chemical environments, immobilizing animals during imaging, quantifying behavior, and automating screens. However, challenges to their widespread adoption in the field include increased complexity over conventional methods, operational problems (such as clogging, leaks, and bubbles), difficulty in obtaining or fabricating devices, and the need to characterize biological results obtained from new assay formats. Here we describe the preparation and operation of simple, reusable microfluidic devices for quantifying behavioral responses to chemical patterns, and single-use devices to arrange animals for time-lapse microscopy and to measure neuronal activity. We focus on details that eliminate or reduce the frustrations commonly experienced by new users of microfluidic devices.
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References
San-Miguel A, Lu H (2013) Microfluidics as a tool for C. elegans research. WormBook, ed. The C. elegans Research Community. http://www.wormbook.org
Chronis N (2010) Worm chips: microtools for C. elegans biology. Lab Chip 10:432–437
Crane MM, Chung K, Stirman J, Lu H (2010) Microfluidics-enabled phenotyping, imaging, and screening of multicellular organisms. Lab Chip 10:1509–1517
Bakhtina NA, Korvink JG (2014) Microfluidic laboratories for C. elegans enhance fundamental studies in biology. RSC Adv 4:4691
Shi W, Wen H, Lin B, Qin J (2011) Microfluidic platform for the study of Caenorhabditis elegans. Top Curr Chem 304:323–338
Albrecht DR, Bargmann CI (2011) High-content behavioral analysis of Caenorhabditis elegans in precise spatiotemporal chemical environments. Nat Methods 8:599–605
Hulme SE, Shevkoplyas SS, Apfeld J et al (2007) A microfabricated array of clamps for immobilizing and imaging C. elegans. Lab Chip 7:1515
Chronis N, Zimmer M, Bargmann CI (2007) Microfluidics for in vivo imaging of neuronal and behavioral activity in Caenorhabditis elegans. Nat Methods 4:727–731
Tian L, Hires SA, Mao T et al (2009) Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators. Nat Methods 6:875–881
Qin D, Xia Y, Whitesides GM (2010) Soft lithography for micro- and nanoscale patterning. Nat Protocols 5:491–502
Mondal S, Ahlawat S, Koushika SP (2012) Simple microfluidic devices for in vivo imaging of C. elegans, Drosophila and zebrafish. J Vis Exp 67, e3780
Gilleland CL, Rohde CB, Zeng F, Yanik MF (2010) Microfluidic immobilization of physiologically active Caenorhabditis elegans. Nat Protoc 5:1888–1902
Larsch J, Ventimiglia D, Bargmann CI, Albrecht DR (2013) High-throughput imaging of neuronal activity in Caenorhabditis elegans. Proc Natl Acad Sci U S A 110:E4266–E4273
Chokshi TV, Bazopoulou D, Chronis N (2010) An automated microfluidic platform for calcium imaging of chemosensory neurons in Caenorhabditis elegans. Lab Chip 10:2758–2763
Haubert K, Drier T, Beebe D (2006) PDMS bonding by means of a portable, low-cost corona system. Lab Chip 6:1548
Ginn BT, Steinbock O (2003) Polymer surface modification using microwave-oven-generated plasma. Langmuir 19:8117–8118
Lee JN, Park C, Whitesides GM (2003) Solvent compatibility of poly(dimethylsiloxane)-based microfluidic devices. Anal Chem 75:6544–6554
Acknowledgements
This work was supported by a Burroughs Wellcome Career Award at the Scientific Interface (DRA). We thank members of the authors’ laboratory, L. Aurilio, K. Tran, K. Burnett, and B. Altshuler for their review and helpful comments while testing these protocols. Strains were obtained from C. Bargmann, V. Ambros, and the CGC.
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Lagoy, R.C., Albrecht, D.R. (2015). Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans . In: Biron, D., Haspel, G. (eds) C. elegans. Methods in Molecular Biology, vol 1327. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4939-2842-2_12
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DOI: https://doi.org/10.1007/978-1-4939-2842-2_12
Publisher Name: Humana Press, Totowa, NJ
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