Abstract
Excessive cell proliferation within atherosclerotic plaques plays an important role in the progression of atherosclerosis. Macrophage proliferation in particular has become a major focus of attention in the cardiovascular field because it appears to mediate most of macrophage expansion in mouse atherosclerotic arteries. Therefore, quantification of cell proliferation is an essential part of the characterization of atherosclerotic plaques in experimental studies. This chapter describes two variants of a simple immunostaining protocol that allow for the quantification of cellular proliferation in mouse atherosclerotic lesions based on the detection of the proliferation-associated antigen Ki-67.
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Acknowledgements
The author is supported by American Heart Association grant 13POST17060028. The author is also grateful to Susan Maclauchlan and Maria A. Zuriaga for insightful discussion and constructive comments on this chapter.
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Fuster, J.J. (2015). Quantification of Cellular Proliferation in Mouse Atherosclerotic Lesions. In: Andrés, V., Dorado, B. (eds) Methods in Mouse Atherosclerosis. Methods in Molecular Biology, vol 1339. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2929-0_14
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DOI: https://doi.org/10.1007/978-1-4939-2929-0_14
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2928-3
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