Abstract
Endothelial cells (ECs) are key blood-vessel-wall components that play critical roles in the regulation of many physiological processes, including angiogenesis, coagulation, and vascular tone control, and in pathological events such as vessel inflammation and leukocyte infiltration. EC dysfunction is one of the first events associated with the development of atherosclerosis and is sustained throughout progression of the disease. The study of ECs in vitro has become an invaluable tool for investigating these vascular processes at the molecular level, and is widely used in the search for therapeutic targets and strategies. This chapter describes a protocol for the isolation and culture of primary mouse aortic ECs based on antibody-mediated EC selection.
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Acknowledgements
We thank Simon Bartlett for English editing. Work in V.A.’s laboratory is supported by grants from the Spanish Ministry for Economy and Competitiveness (MINECO) (SAF2013-46663-R), the Fondo Europeo de Desarrollo Regional (FEDER), the Instituto de Salud Carlos III (RD12/0042/0028), the Progeria Research Foundation (Innovator Award 2012, Established Investigator Award 2014), and the European Union (Liphos, Grant Agreement 317916). P.M-S. is supported by an FPU predoctoral fellowship from MINECO. The Centro Nacional de Investigaciones Cardiovasculares (CNIC) is supported by the MINECO and the Pro-CNIC Foundation.
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Molina-Sánchez, P., Andrés, V. (2015). Isolation of Mouse Primary Aortic Endothelial Cells by Selection with Specific Antibodies. In: Andrés, V., Dorado, B. (eds) Methods in Mouse Atherosclerosis. Methods in Molecular Biology, vol 1339. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2929-0_7
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DOI: https://doi.org/10.1007/978-1-4939-2929-0_7
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2928-3
Online ISBN: 978-1-4939-2929-0
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