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The Role of Ubiquitination to Determine Non-Smad Signaling Responses

  • Protocol
TGF-β Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1344))

Abstract

Ubiquitination is a posttranslational modification of proteins which acts as a key regulator of their function as well as fate. We have recently reported transforming growth factor β (TGFβ)-induced activation of non-Smad signaling responses through a specific Lys63-linked polyubiquitination of TGFβ type I receptor and TGFβ-associated kinase 1 (TAK1) that are utilized to specify cellular responses in cancer cells. This chapter gives a brief introduction of the biological importance of ubiquitination of proteins, the methods we have used for detecting new partners in the TGFβ signaling pathway and for performing ubiquitination assays.

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Acknowledgements

The authors thank Susanne Grimsby, Alessandro Sorrentino, Reshma Sundar, and Noopur Thakur for their contributions to the original research. We thank Carl-Henrik Heldin and our colleges at the Ludwig Institute for Cancer Research, Uppsala Branch, for valuable discussions. This work was supported by the Swedish Medical Research Council, the Swedish Cancer Society, the Torsten and Ragnar Söderbergs Foundation, Umeå University, Cancer Research Foundation Norrland and a regional agreement between Umeå University and Västerbotten County Council on cooperation in the field of Medicine, Odontology and Health (ALF).

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Authors and Affiliations

  1. Department of Medical Biosciences, Umeå University, Pathology Building 6M, 2nd Floor, Umeå, 901 85, Sweden

    Shyam Kumar Gudey & Marene Landström Ph.D.

Authors
  1. Shyam Kumar Gudey
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  2. Marene Landström Ph.D.
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Corresponding author

Correspondence to Marene Landström Ph.D. .

Editor information

Editors and Affiliations

  1. Departments of Surgery and Molecular & Cellular Biology, Baylor College of Medicine, Houston, Texas, USA

    Xin-Hua Feng

  2. Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang Proviince, China

    Pinglong Xu

  3. Department of Surgery, Baylor College of Medicine, Houston, Texas, USA

    Xia Lin

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Gudey, S.K., Landström, M. (2016). The Role of Ubiquitination to Determine Non-Smad Signaling Responses. In: Feng, XH., Xu, P., Lin, X. (eds) TGF-β Signaling. Methods in Molecular Biology, vol 1344. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2966-5_23

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  • DOI: https://doi.org/10.1007/978-1-4939-2966-5_23

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2965-8

  • Online ISBN: 978-1-4939-2966-5

  • eBook Packages: Springer Protocols

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