Abstract
HLA-G is a nonclassical human leukocyte antigen (HLA) class I molecule which plays important tolerogenic functions in various physiological and pathological situations such as fetus and transplant acceptance, and immune escaping of virus-infected and malignant cells. Here we describe a method, which allows for studying cell surface expression of HLA-G using specific antibodies with flow cytometry analysis.
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References
Kovats S, Main EK, Librach C et al (1990) A class I antigen, HLA-G, expressed in human trophoblasts. Science 248:220–223
Yan WH (2011) Human leukocyte antigen-G in cancer: are they clinically relevant? Cancer Lett 311:123–130
Yan WH (2011) HLA-G expression in cancers: potential role in diagnosis, prognosis and therapy. Endocr Metab Immune Disord Drug Targets 11:76–89
Fainardi E, Castellazzi M, Stignani M et al (2011) Emerging topics and new perspectives on HLA-G. Cell Mol Life Sci 68:433–451
González A, Rebmann V, LeMaoult J et al (2012) The immunosuppressive molecule HLA-G and its clinical implications. Crit Rev Clin Lab Sci 49:63–84
Carosella ED, Gregori S, LeMaoult J (2011) The tolerogenic interplay(s) among HLA-G, myeloid APCs, and regulatory cells. Blood 118:6499–6505
Naji A, Menier C, Morandi F et al (2014) Binding of HLA-G to ITIM-bearing Ig-like transcript 2 receptor suppresses B cell responses. J Immunol 192:1536–1546
Baudhuin J, Migraine J, Faivre V et al (2013) Exocytosis acts as a modulator of the ILT4-mediated inhibition of neutrophil functions. Proc Natl Acad Sci U S A 110:17957–17962
Brown R, Kabani K, Favaloro J et al (2012) CD86+ or HLA-G+ can be transferred via trogocytosis from myeloma cells to T cells and are associated with poor prognosis. Blood 120:2055–2063
Carosella ED (2011) The tolerogenic molecule HLA-G. Immunol Lett 138:22–24
Paul P, Rouas-Freiss N, Moreau P et al (2000) HLA-G, -E, -F preworkshop: tools and protocols for analysis of non-classical class I genes transcription and protein expression. Hum Immunol 61:1177–1195
LeMaoult J, Le Discorde M, Rouas-Freiss N et al (2003) Biology and functions of human leukocyte antigen-G in health and sickness. Tissue Antigens 62:273–284
Chen BG, Xu DP, Lin A et al (2013) NK cytolysis is dependent on the proportion of HLA-G expression. Hum Immunol 74:286–289
Acknowledgements
This work is supported by grants from National Natural Science Foundation of China (31170879, 31370920, 81372247), Zhejiang Provincial Natural Science Foundation of China (LR13H160001), and by Zhejiang Provincial program for the cultivation of high-level innovative health talents.
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Lin, A., Yan, WH. (2016). HLA-G as an Inhibitor of Immune Responses. In: Cuturi, M., Anegon, I. (eds) Suppression and Regulation of Immune Responses. Methods in Molecular Biology, vol 1371. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3139-2_1
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DOI: https://doi.org/10.1007/978-1-4939-3139-2_1
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3138-5
Online ISBN: 978-1-4939-3139-2
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