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PTEN at 18: Still Growing

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PTEN

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1388))

Abstract

Discovered in 1997, PTEN remains one of the most studied tumor suppressors. In this issue of Methods in Molecular Biology, we assembled a series of papers describing various clinical and experimental approaches to studying PTEN function. Due to its broad expression, regulated subcellular localization, and intriguing phosphatase activity, methodologies aimed at PTEN study have often been developed in the context of mutations affecting various aspects of its regulation, found in patients burdened with PTEN loss-driven tumors. PTEN’s extensive posttranslational modifications and dynamic localization pose unique challenges for studying PTEN features in isolation and necessitate considerable development of experimental systems to enable controlled characterization. Nevertheless, ongoing efforts towards the development of PTEN knockout and knock-in animals and cell lines, antibodies, and enzymatic assays have facilitated a huge body of work, which continues to unravel the fascinating biology of PTEN.

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Acknowledgements

The work in the authors’ laboratory is supported by the funds from the Canadian Institutes of Health Research and the Canadian Cancer Society. OG is supported by the Excellence in Radiation Research for the 21st century (EIRR21st) postdoctoral fellowship.

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Authors and Affiliations

  1. Princess Margaret Cancer Centre, University Health Network, Room 13-313, Toronto Medical Discovery Tower, 101 College Street, Toronto, M5G 1L7, ON, Canada

    Olena Gorbenko

  2. University of Toronto, Princess Margaret Cancer Centre, Room 13-313, Toronto Medical Discovery Tower, 101 College Street, Toronto, M5G 1L7, ON, Canada

    Vuk Stambolic

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  1. Olena Gorbenko
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  2. Vuk Stambolic
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Correspondence to Vuk Stambolic .

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Editors and Affiliations

  1. University of Toronto, Princess Margaret Cancer Centre, Toronto, Ontario, Canada

    Leonardo Salmena

  2. University of Toronto, Princess Margaret Cancer Centre, Toronto, Ontario, Canada

    Vuk Stambolic

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Gorbenko, O., Stambolic, V. (2016). PTEN at 18: Still Growing. In: Salmena, L., Stambolic, V. (eds) PTEN. Methods in Molecular Biology, vol 1388. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3299-3_2

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  • DOI: https://doi.org/10.1007/978-1-4939-3299-3_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3297-9

  • Online ISBN: 978-1-4939-3299-3

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