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Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics

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Clinical Applications of PCR

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1392))

Abstract

Tissues are heterogeneous in their components. If cells of interest are a minor population of collected tissue, it would be difficult to obtain genetic or genomic information of the interested cell population with conventional genomic DNA extraction from the collected tissue. Single-cell DNA analysis is important in the analysis of genetics of cell clonality, genetic anticipation, and single-cell DNA polymorphisms. Single-cell PCR using Single Cell Ampligrid/GeXP platform is described in this chapter.

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Author information

Authors and Affiliations

  1. Department of Hematopathology, The University of Texas MD Anderson Cancer Center, 8515 Fannin Street, Houston, TX, 77054, USA

    Chi Young Ok, Rajesh R. Singh & Alaa A. Salim

Authors
  1. Chi Young Ok
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  2. Rajesh R. Singh
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  3. Alaa A. Salim
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Corresponding author

Correspondence to Rajesh R. Singh .

Editor information

Editors and Affiliations

  1. Anderson Cancer Center, The University of Texas MD, Houston, Texas, USA

    Rajyalakshmi Luthra

  2. Anderson Cancer Center, The University of Texas MD, Houston, Texas, USA

    Rajesh R. Singh

  3. Anderson Cancer Center, The University of Texas MD, Houston, Texas, USA

    Keyur P. Patel

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© 2016 Springer Science+Business Media New York

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Ok, C.Y., Singh, R.R., Salim, A.A. (2016). Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics. In: Luthra, R., Singh, R., Patel, K. (eds) Clinical Applications of PCR. Methods in Molecular Biology, vol 1392. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3360-0_14

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  • DOI: https://doi.org/10.1007/978-1-4939-3360-0_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3358-7

  • Online ISBN: 978-1-4939-3360-0

  • eBook Packages: Springer Protocols

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