Abstract
Endogenous retroviruses are repetitive sequences found abundantly in mammalian genomes which are capable of modulating host gene expression. Nevertheless, most endogenous retrovirus copies are under tight epigenetic control via histone-repressive modifications and DNA methylation. Here we describe a common method used in our laboratory to detect, quantify, and compare mammalian endogenous retrovirus DNA methylation. More specifically we describe methylated DNA immunoprecipitation (MeDIP) followed by quantitative PCR.
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Rebollo, R., Mager, D.L. (2016). Methylated DNA Immunoprecipitation Analysis of Mammalian Endogenous Retroviruses. In: Garcia-Pérez, J. (eds) Transposons and Retrotransposons. Methods in Molecular Biology, vol 1400. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3372-3_23
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DOI: https://doi.org/10.1007/978-1-4939-3372-3_23
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3370-9
Online ISBN: 978-1-4939-3372-3
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