Abstract
Outer membrane proteins (OMPs) are unique to Gram-negative bacteria and have been revealed as potential vaccine candidates for conferring protection against infections in recent years. Immunoproteomics is a powerful technique that is ideally suited to screen and identify potential vaccine candidates. This chapter presents a brief outline of the screening of immunogenic OMPs from Vibrio parahaemolyticus by an immunoproteomic strategy that was based on two-dimensional electrophoresis (2-DE) and immunoblotting. The protective efficacy provided by the immunogenic OMP Vp0802 determined by active protection experiment assays is also presented in brief.
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Acknowledgements
This work was supported by grants from the NSFC (31200110), the Natural Science Foundation of Zhejiang Province (LY15C010004), the 521 Talent Program of Zhejiang Sci-Tech University to J. Pan.
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Pan, J., Li, C., Ye, Z. (2016). Immunoproteomic Approach for Screening Vaccine Candidates from Bacterial Outer Membrane Proteins. In: Thomas, S. (eds) Vaccine Design. Methods in Molecular Biology, vol 1404. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-3389-1_34
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DOI: https://doi.org/10.1007/978-1-4939-3389-1_34
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