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Breast Cancer Stem Cell Isolation

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Breast Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1406))

Abstract

Cells within the tumor are highly heterogeneous. Only a small portion of the cells within the tumor is capable to generate a new tumor. These cells are called cancer stem cells. Theoretically, cancer stem cells are originally from normal stem cells or early progenitor cells which accumulate the random mutations and undergo an altered version of the normal differentiation process. The cancer stem cell drives tumor progression and its recurrence. Thus, the technique to identify and purify the cancer stem cell is the key in any cancer stem cell research. In this protocol, we provide the basic technology of identification and purification of breast cancer stem cells as well as further functional assays to help the researchers achieve their research goals.

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Acknowledgements

This work was supported in part by grants from NIH R01CA70896, R01CA75503, R01CA86072 (R.G.P.), the Breast Cancer Research Foundation (R.G.P.), and the Department of Defense Concept Award W81XWH-1101-0303. The Sidney Kimmel Cancer Center was supported by the NIH Cancer Center Core Grant P30CA56036 (R.G.P). This project is funded in part by the Dr. Ralph and Marian C. Falk Medical Research Trust (R.G.P.), grants from the Pennsylvania Department of Health (R.G.P.). The Department specifically disclaims responsibility for analyses, interpretations, or conclusions. There are no conflicts of interest associated with this manuscript.

Conflicts of Interest: RGP is the founder of ProstaGene, LLC and owns patents related to prostate cancer cell lines and their uses thereof.

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    Authors and Affiliations

    1. Department of Cancer Biology, Sidney Kimmel Cancer Center, Thomas Jefferson University, Bluemle Life Sciences Building, 233 South 10th Street, Philadelphia, PA, 19107, USA

      Xuanmao Jiao, Massimo Cristofanilli & Richard G. Pestell

    2. Kazan Federal University, 18 Kremlyovskaya St, Kazan, Republic of Tatarstan, 420008, Russia

      Albert A. Rizvanov, Regina R. Miftakhova & Richard G. Pestell

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    1. Xuanmao Jiao
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    2. Albert A. Rizvanov
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    3. Massimo Cristofanilli
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    4. Regina R. Miftakhova
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    5. Richard G. Pestell
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    Correspondence to Richard G. Pestell .

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    Editors and Affiliations

    1. School of Medicine, Stony Brook University, Stony Brook, New York, USA

      Jian Cao

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    Jiao, X., Rizvanov, A.A., Cristofanilli, M., Miftakhova, R.R., Pestell, R.G. (2016). Breast Cancer Stem Cell Isolation. In: Cao, J. (eds) Breast Cancer. Methods in Molecular Biology, vol 1406. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3444-7_10

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    • DOI: https://doi.org/10.1007/978-1-4939-3444-7_10

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    • Publisher Name: Humana Press, New York, NY

    • Print ISBN: 978-1-4939-3442-3

    • Online ISBN: 978-1-4939-3444-7

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