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Clinical-Grade Manufacturing of Therapeutic Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Culture Systems

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Mesenchymal Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1416))

Abstract

The therapeutic potential of mesenchymal stem/stromal cells (MSC) has triggered the need for high cell doses in a vast number of clinical applications. This demand requires the development of good manufacturing practices (GMP)-compliant ex vivo expansion protocols that should be effective to deliver a robust and reproducible supply of clinical-grade cells in a safe and cost-effective manner. Controlled stirred-tank bioreactor systems under xenogeneic (xeno)-free culture conditions offer ideal settings to develop and optimize cell manufacturing to meet the standards and needs of human MSC for cellular therapies. Herein we describe two microcarrier-based stirred culture systems using spinner flasks and controlled stirred-tank bioreactors under xeno-free conditions for the efficient ex vivo expansion of human bone marrow and adipose tissue-derived MSC.

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Acknowledgments

The authors greatly acknowledge Jeffrey M. Gimble, M.D. (Center for Stem Cell Research and Regenerative Medicine, Tulane University, New Orleans, Louisiana, USA) for kindly providing the human adipose-derived stem/stromal cells. The authors also thank the financial support from Fundação para a Ciência e a Tecnologia (FCT), Portugal, through iBB - Institute for Bioengineering and Biosciences under the project UID/BIO/04565/2013 and Programa Operacional Regional de Lisboa 2020 (Project N. 007317), projects PTDC/EQU-EQU/114231/2009 and HMSP-ICT/0001/2011 and grant SFRH/BPD/82062/2011 (awarded to AFP).

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Authors and Affiliations

  1. Department of Bioengineering and iBB - Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Avenida Rovisco Pais, Lisboa, 1049-001, Portugal

    Ana Fernandes-Platzgummer, Joana G. Carmelo, Cláudia Lobato da Silva & Joaquim M. S. Cabral

Authors
  1. Ana Fernandes-Platzgummer
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  2. Joana G. Carmelo
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  3. Cláudia Lobato da Silva
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  4. Joaquim M. S. Cabral
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Corresponding author

Correspondence to Cláudia Lobato da Silva .

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Editors and Affiliations

  1. Dept of Molecular Med,Unit of Cardiology, University of Pavia, PAVIA, Italy

    Massimiliano Gnecchi

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Fernandes-Platzgummer, A., Carmelo, J.G., da Silva, C.L., Cabral, J.M.S. (2016). Clinical-Grade Manufacturing of Therapeutic Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Culture Systems. In: Gnecchi, M. (eds) Mesenchymal Stem Cells. Methods in Molecular Biology, vol 1416. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3584-0_22

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  • DOI: https://doi.org/10.1007/978-1-4939-3584-0_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3582-6

  • Online ISBN: 978-1-4939-3584-0

  • eBook Packages: Springer Protocols

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