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Whole-Cell Patch-Clamp Recording of Mouse and Rat Inner Hair Cells in the Intact Organ of Corti

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Auditory and Vestibular Research

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1427))

Abstract

Whole-cell patch clamping is a widely applied method to record currents across the entire membrane of a cell. This protocol describes application of this method to record currents from the sensory inner hair cells in the intact auditory sensory epithelium, the organ of Corti, isolated from rats or mice. This protocol particularly outlines the basic equipment required, provides instructions for the preparation of solutions and small equipment items, and methodology for recording voltage-activated and evoked synaptic currents from the inner hair cells.

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Author information

Authors and Affiliations

  1. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (CONICET), Vuelta de Obligado 2490, C. A., Buenos Aires, 1428, Argentina

    Juan D. Goutman

  2. Department of Otorhinolaryngology, University Medical Center Groningen, Hanzeplein 1, Groningen, GZ, 9713, The Netherlands

    Sonja J. Pyott

Authors
  1. Juan D. Goutman
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  2. Sonja J. Pyott
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Corresponding author

Correspondence to Sonja J. Pyott .

Editor information

Editors and Affiliations

  1. College of Medicine, University of South Florida, Tampa, Florida, USA

    Bernd Sokolowski

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Goutman, J.D., Pyott, S.J. (2016). Whole-Cell Patch-Clamp Recording of Mouse and Rat Inner Hair Cells in the Intact Organ of Corti. In: Sokolowski, B. (eds) Auditory and Vestibular Research. Methods in Molecular Biology, vol 1427. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3615-1_26

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  • DOI: https://doi.org/10.1007/978-1-4939-3615-1_26

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3613-7

  • Online ISBN: 978-1-4939-3615-1

  • eBook Packages: Springer Protocols

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