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Modeling Leukemogenesis in the Zebrafish Using Genetic and Xenograft Models

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Zebrafish

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1451))

Abstract

The zebrafish is a widely accepted model to study leukemia. The major advantage of studying leukemogenesis in zebrafish is attributed to its short life cycle and superior imaging capacity. This chapter highlights using transgenic- and xenograft-based models in zebrafish to study a specific leukemogenic mutation and analyze therapeutic responses in vivo.

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Acknowledgments

The authors would like to acknowledge members of Berman and Dellaire lab especially Dale Corkery, Chansey Veinotte, Sergey Prykhozhij, Nicole Melong, Babak Razaghi, Jaime Wertman, and Victoria Bentley for the critical review. Vinothkumar Rajan is funded through Cancer Research Trainee Program of the Beatrice Hunter Cancer Institute, Nova Scotia in partnership with the Canadian Imperial bank of Commerce and New Brunswick Health Research Foundation.

Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, Dalhousie University, Halifax, Canada, B3H 4R2

    Vinothkumar Rajan & Jason N. Berman

  2. Department of Pathology, Dalhousie University, 5850/5980 University Ave, Halifax, Canada, B3H 4R2

    Graham Dellaire & Jason N. Berman

  3. Department of Biochemistry & Molecular Biology, Dalhousie University, Halifax, Canada, B3H 4R2

    Graham Dellaire

  4. Department of Pediatrics, IWK Health Centre/Dalhousie University, Halifax, Canada, B3H 4R2

    Jason N. Berman

Authors
  1. Vinothkumar Rajan
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  2. Graham Dellaire
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  3. Jason N. Berman
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Corresponding author

Correspondence to Jason N. Berman .

Editor information

Editors and Affiliations

  1. Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Japan

    Koichi Kawakami

  2. Institute of Genetics and Molecular Medicine, MRC Human Genetics Unit & The University of Edinburgh Cancer Research UK Centre, Edinburgh, United Kingdom

    E. Elizabeth Patton

  3. Champalimaud Centre for the Unknown, Champalimaud Neuroscience Programme Champalimaud Centre for the Unknown, Lisbon, Portugal

    Michael Orger

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Rajan, V., Dellaire, G., Berman, J.N. (2016). Modeling Leukemogenesis in the Zebrafish Using Genetic and Xenograft Models. In: Kawakami, K., Patton, E., Orger, M. (eds) Zebrafish. Methods in Molecular Biology, vol 1451. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3771-4_12

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  • DOI: https://doi.org/10.1007/978-1-4939-3771-4_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3769-1

  • Online ISBN: 978-1-4939-3771-4

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