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Analysis of Endogenous Protein Interactions of Polycomb Group of Proteins in Mouse Embryonic Stem Cells

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Polycomb Group Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1480))

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Abstract

PRC1 complexes contain four core subunits: Pcgf, Phc, Ring1, and Cbx proteins. Interestingly, mammalian genomes have several paralogues for each subunit, which are differentially expressed depending on the cell type, differentiation program, and cellular stimuli. Therefore, identification and characterization of the specific architecture of different PRC1 complexes during cellular differentiation are essential to better understand the function and recruitment mechanism of PRC1 complexes. In this chapter we describe several methods to study Polycomb architecture, and identification of novel interactors in both pluripotent and differentiating mouse embryonic stem cells.

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Author information

Authors and Affiliations

  1. Gene Regulation, Stem Cells and Cancer Programme, Centre for Genomic Regulation (CRG), Dr Aiguader 88, 08003, Barcelona, Spain

    Lluis Morey & Luciano Di Croce

  2. Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, 1475 N.W. 12th Avenue, Miami, FL, 33136, USA

    Lluis Morey

  3. Institució Catalana de Recerca i Estudis Avançats, Pg. Lluis Companys 23, 08010, Barcelona, Spain

    Luciano Di Croce

Authors
  1. Lluis Morey
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  2. Luciano Di Croce
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Corresponding author

Correspondence to Lluis Morey .

Editor information

Editors and Affiliations

  1. IBCN-CNR, Institute of Cell Biology and Neurobiology and Istituto Nazionale di Genetica Molecolare “Romeo ed Enrica Invernizzi”, Rome, Italy

    Chiara Lanzuolo

  2. Istituto Nazionale di Genetica Molecolare “Romeo ed Enrica Invernizzi”, Milan, Italy

    Beatrice Bodega

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Morey, L., Di Croce, L. (2016). Analysis of Endogenous Protein Interactions of Polycomb Group of Proteins in Mouse Embryonic Stem Cells. In: Lanzuolo, C., Bodega, B. (eds) Polycomb Group Proteins. Methods in Molecular Biology, vol 1480. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6380-5_14

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  • DOI: https://doi.org/10.1007/978-1-4939-6380-5_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6378-2

  • Online ISBN: 978-1-4939-6380-5

  • eBook Packages: Springer Protocols

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