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Isolation of Plastid Ribosomes

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Isolation of Plant Organelles and Structures

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1511))

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Abstract

Plastid ribosomes are responsible for a large part of the protein synthesis in plant leaves, green algal cells, and the vast majority in the thalli of red algae. Plastid translation is necessary not only for photosynthesis but also for development/differentiation of plants and algae. While some isolated plastid ribosomes from a few green lineages have been characterized by biochemical and proteomic approaches, in-depth proteomics including analyses of posttranslational modifications and processing, comparative proteomics of plastid ribosomes isolated from the cells grown under different conditions, and those from different taxa are still to be carried out. Establishment of isolation methods for pure plastid ribosomes from a wider range of species would be beneficial to study the relationship between structure, function, and evolution of plastid ribosomes. Here I describe methodologies and provide example protocols for extraction and isolation of plastid ribosomes from a unicellular green alga (Chlamydomonas reinhardtii), a land plant (Arabidopsis thaliana), and a marine red macroalga (Pyropia yezoensis).

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Acknowledgements

I thank Dr. Alap. R. Subramanian, Dr. Stephen. P. Mayfield, Dr. Don. P. Bourque, Dr. Tatsuya Oda, Dr. Kenji Hara, Dr. Kazuyoshi Kuwano, Dr. Masahiro Kobayashi, and Dr. Yuji Fujita for their support, comments, and advice. I am grateful to Dr. Nicolas Taylor and Dr. Harvey Millar for their helpful comments and suggestions on the manuscript. I also thank Ms. Moemi Yamawaki for technical assistance with preparation and proteomic characterization of plastid ribosomes from Pyropia yezoensis. This work was supported by funds from the Grant-in-Aid from the Japan Society for the Promotion of Science (24580303 and 15K07582) to KY.

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Authors and Affiliations

  1. Division of Biochemistry, Faculty of Fisheries, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan

    Kenichi Yamaguchi

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  1. Kenichi Yamaguchi
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Correspondence to Kenichi Yamaguchi .

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Editors and Affiliations

  1. The University of Western Australia, Perth, West Australia, Australia

    Nicolas L. Taylor

  2. The University of Western Australia, Perth, West Australia, Australia

    A. Harvey Millar

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Yamaguchi, K. (2017). Isolation of Plastid Ribosomes. In: Taylor, N., Millar, A. (eds) Isolation of Plant Organelles and Structures. Methods in Molecular Biology, vol 1511. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6533-5_20

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  • DOI: https://doi.org/10.1007/978-1-4939-6533-5_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6531-1

  • Online ISBN: 978-1-4939-6533-5

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