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Agrobacterium-Mediated Transformation of Leaf Base Segments

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Oat

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1536))

Abstract

Agrobacterium-mediated transformation has become a routine method of genetic engineering of cereals, gradually replacing the biolistic protocols. Simple integration patterns of transgenic loci, decent transformation efficiency, and technical simplicity are the main advantages offered by this method. Here we present a detailed protocol for the production of transgenic oat plants by Agrobacterium-mediated transformation of leaf base segments. The use of leaf explants as target tissues for transformation and in vitro regeneration of transgenic plants may be a good alternative for genotypes which are not susceptible to regeneration from immature or mature embryos. We also describe the biochemical and molecular analysis procedures of the transgenic plants including a GUS histochemical assay, and Southern blot, both of which are optimized for application in oat.

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Acknowledgment

This research was supported by the Statutory Funds of Plant Breeding and Acclimatization Institute—National Research Institute, No. 1-1-01-4-04.

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Authors and Affiliations

  1. Department of Functional Genomics, Plant Breeding and Acclimatization Institute—National Research Institute, Radzików, 05-870, Błonie, Poland

    Sebastian Gasparis

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  1. Sebastian Gasparis
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Correspondence to Sebastian Gasparis .

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  1. Department of Functional Genomics, Plant Breeding & Acclimatization Inst. National Research Institute, Blonie, Poland

    Sebastian Gasparis

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Gasparis, S. (2017). Agrobacterium-Mediated Transformation of Leaf Base Segments. In: Gasparis, S. (eds) Oat. Methods in Molecular Biology, vol 1536. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6682-0_7

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  • DOI: https://doi.org/10.1007/978-1-4939-6682-0_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6680-6

  • Online ISBN: 978-1-4939-6682-0

  • eBook Packages: Springer Protocols

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