Skip to main content
SpringerLink
Log in

Using Confocal Microscopy and Computational Modeling to Investigate the Cell-Penetrating Properties of Antimicrobial Peptides

  • Protocol
  • First Online:
Antimicrobial Peptides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1548))

Abstract

Antimicrobial peptides (AMPs) may display the ability to penetrate cells, which may be relevant for their antibiotic activity. To investigate the relevance of the penetrating activity for the antibiotic activity of AMPs, here we describe a method based on the combined use of confocal microscopy and computational modeling coupled with cell death kinetics.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Wang G, Li X, Wang Z (2009) APD2: the updated antimicrobial peptide database and its application in peptide design. Nucleic Acids Res 37:D933–D937

    Article  CAS  PubMed  Google Scholar 

  2. Seo MD, Won HS, Kim JH, Mishig-Ochir T, Lee BJ (2012) Antimicrobial peptides for therapeutic applications: a review. Molecules 17:12276–12286

    Article  CAS  PubMed  Google Scholar 

  3. Splith K, Neundorf I (2011) Antimicrobial peptides with cell-penetrating peptide properties and vice versa. Eur Biophys J 40:387–397

    Article  CAS  PubMed  Google Scholar 

  4. Falco A, Ortega-Villaizan M, Chico V, Brocal I, Perez L, Coll JM, Estepa A (2009) Antimicrobial peptides as model molecules for the development of novel antiviral agents in aquaculture. Mini Rev Med Chem 9:1159–1164

    Article  CAS  PubMed  Google Scholar 

  5. Haney EF, Hancock RE (2013) Peptide design for antimicrobial and immunomodulatory applications. Biopolymers 100:572–583

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Henriques ST, Melo MN, Castanho MA (2006) Cell-penetrating peptides and antimicrobial peptides: how different are they? Biochem J 399:1–7

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Palm C, Netzereab S, Hällbrink M (2006) Quantitatively determined uptake of cell-penetrating peptides in non-mammalian cells with an evaluation of degradation and antimicrobial effects. Peptides 27:1710–1716

    Article  CAS  PubMed  Google Scholar 

  8. Matsuzaki K, Murase O, Fujii N, Miyajima K (1995) Translocation of a channel-forming antimicrobial peptide, magainin 2, across lipid bilayers by forming a pore. Biochemistry 34:6521–6526

    Article  CAS  PubMed  Google Scholar 

  9. Otvos L, Cudic M (2007) Broth microdilution antibacterial assay of peptides. Methods Mol Biol 386:309–320

    CAS  PubMed  Google Scholar 

  10. Jorgensen JH, Ferraro MJ (2009) Antimicrobial susceptibility testing: a review of general principles and contemporary practices. Clin Infect Dis 49:1749–1755

    Article  CAS  PubMed  Google Scholar 

  11. Gee ML, Burton M, Grevis-James A, Hossain MA, McArthur S, Palombo EA, Wade JD, Clayton AH (2013) Imaging the action of antimicrobial peptides on living bacterial cells. Sci Rep 3:1557

    Article  PubMed  PubMed Central  Google Scholar 

  12. Marchand C, Krajewski K, Lee HF, Antony S, Johnson AA, Amin R, Roller P, Kvaratskhelia M, Pommier Y (2006) Covalent binding of the natural antimicrobial peptide indolicidin to DNA abasic sites. Nucleic Acids Res 34:5157–5165

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Shai Y (2002) Mode of action of membrane active antimicrobial peptides. Biopolymers 66:236–248

    Article  CAS  PubMed  Google Scholar 

  14. Bechara C, Sagan S (2013) Cell-penetrating peptides: 20 years later, where do we stand? FEBS Lett 587:1693–1702

    Article  CAS  PubMed  Google Scholar 

  15. Rodriguez Plaza JG, Morales-Nava R, Diener C, Schreiber G, Gonzalez ZD, Lara Ortiz MT, Ortega Blake I, Pantoja O, Volkmer R, Klipp E, Herrmann A, Del Rio G (2014) Cell penetrating peptides and cationic antibacterial peptides: two sides of the same coin. J Biol Chem 289:14448–14457

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Rodríguez Plaza JG, Villalón Rojas A, Herrera S, Garza-Ramos G, Torres Larios A, Amero C, Zarraga Granados G, Gutiérrez Aguilar M, Lara Ortiz MT, Polanco Gonzalez C, Uribe Carvajal S, Coria R, Peña Díaz A, Bredesen DE, Castro-Obregon S, del Rio G (2012) Moonlighting peptides with emerging function. PLoS One 7:e40125

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgment

We would like to thank Dr. Maria Teresa Lara Ortiz for her technical assistance in the writing of this work. Grants from the Alexander von Humboldt Foundation (FKZ: 3.4-IP-DEU/113263) and PAPIIT (IN205911) to GDR supported this work.

Author information

Authors and Affiliations

  1. Biochemistry and Structural Biology Department, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Circuito Exterior S/N Ciudad Universitaria, México D.F., 04510, Mexico

    Gabriel Del Rio

  2. Theoretische und Molekulare Biophysik, Humboldt-Universität zu Berlin, Berlin, Germany

    Edda Klipp & Andreas Herrmann

Authors
  1. Gabriel Del Rio
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Edda Klipp
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Andreas Herrmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gabriel Del Rio .

Editor information

Editors and Affiliations

  1. Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

    Paul R. Hansen

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Science+Business Media LLC

About this protocol

Cite this protocol

Del Rio, G., Klipp, E., Herrmann, A. (2017). Using Confocal Microscopy and Computational Modeling to Investigate the Cell-Penetrating Properties of Antimicrobial Peptides. In: Hansen, P. (eds) Antimicrobial Peptides. Methods in Molecular Biology, vol 1548. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6737-7_13

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-6737-7_13

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6735-3

  • Online ISBN: 978-1-4939-6737-7

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation