Abstract
Autophagy is a highly conserved cellular mechanism of “self digestion,” ensuring cellular homeostasis, and playing a role in many diseases including cancer. As a stress response mechanism, it may also be involved in cellular response to therapy.
LC3 and Sequestosome 1 (p62/SQSTM1) are among the most widely used markers to monitor autophagy, and can be visualized in formalin-fixed and paraffin-embedded tissue by immunohistochemistry. Here we describe a validated staining protocol using an automated staining system available in many routine pathology laboratories, enabling high-throughput staining under standardized conditions.
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Acknowledgment
This work was supported by a grant from the Bernese Cancer League to SB.
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Berezowska, S., Galván, J.A. (2017). Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue. In: Pellicciari, C., Biggiogera, M. (eds) Histochemistry of Single Molecules. Methods in Molecular Biology, vol 1560. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6788-9_13
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DOI: https://doi.org/10.1007/978-1-4939-6788-9_13
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6787-2
Online ISBN: 978-1-4939-6788-9
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