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The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium

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Histochemistry of Single Molecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1560))

Abstract

Lectin histochemistry is commonly used to characterize the pattern of glycoconjugates in cells and tissues. Recent studies show that alterations in these glycoconjugates are associated with the entry of cells into apoptosis. A widely used technique for the detection of apoptotic cell death is TUNEL. In this chapter, we study the sensitivity of both techniques to identify apoptotic cells in the testis of photo-inhibited Syrian hamster.

An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-6788-9_26

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Acknowledgment

This work was supported by GERM 19892/15 from Fundación Seneca CARM.

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Authors and Affiliations

  1. Department of Cell Biology and Histology, School of Medicine, IMIB-Arrixaca, University of Murcia, Regional Campus of International Excellence, Campus Mare Nostrum, Espinardo, Murcia, 30100, Spain

    Vicente Seco-Rovira, Ester Beltrán-Frutos, Jesús Martínez-Hernández, Concepción Ferrer & Luis Miguel Pastor

Authors
  1. Vicente Seco-Rovira
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  2. Ester Beltrán-Frutos
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  3. Jesús Martínez-Hernández
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  4. Concepción Ferrer
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  5. Luis Miguel Pastor
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Corresponding author

Correspondence to Luis Miguel Pastor .

Editor information

Editors and Affiliations

  1. Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

    Carlo Pellicciari

  2. Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

    Marco Biggiogera

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Seco-Rovira, V., Beltrán-Frutos, E., Martínez-Hernández, J., Ferrer, C., Pastor, L.M. (2017). The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium. In: Pellicciari, C., Biggiogera, M. (eds) Histochemistry of Single Molecules. Methods in Molecular Biology, vol 1560. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6788-9_9

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  • DOI: https://doi.org/10.1007/978-1-4939-6788-9_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6787-2

  • Online ISBN: 978-1-4939-6788-9

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