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Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors

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Protein Terminal Profiling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1574))

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Abstract

In this chapter, you will learn how to use translocation biosensors to investigate protease functions in living cells. We here present modular protein translocation biosensors tailored to investigate protease activity and protein-protein interactions. Besides the mapping of protease function, the biosensors are also applicable to identify chemicals and/or (nano)materials modulating the respective protein activities and can also be exploited for RNAi-mediated genetic screens.

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Authors and Affiliations

  1. Molecular and Cellular Oncology, ENT/University Medical Center Mainz, Langenbeckstr. 1, 55131, Mainz, Germany

    Angelina Hahlbrock, Dorothée Gößwein & Roland H. Stauber

Authors
  1. Angelina Hahlbrock
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  2. Dorothée Gößwein
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  3. Roland H. Stauber
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Corresponding author

Correspondence to Angelina Hahlbrock .

Editor information

Editors and Affiliations

  1. Institute of Molecular Medicine and Cell Research, University Freiburg, Freiburg, Germany

    Oliver Schilling

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Hahlbrock, A., Gößwein, D., Stauber, R.H. (2017). Protein Translocation Assays to Probe Protease Function and Screen for Inhibitors. In: Schilling, O. (eds) Protein Terminal Profiling. Methods in Molecular Biology, vol 1574. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6850-3_17

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  • DOI: https://doi.org/10.1007/978-1-4939-6850-3_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6849-7

  • Online ISBN: 978-1-4939-6850-3

  • eBook Packages: Springer Protocols

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