Abstract
In this chapter a detailed description of the fabrication and testing of an aptasensor for influenza A virus detection is given. The sensor chip is an all-polymer chip fabricated with screen-printed poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) electrodes. Chip substrates are made by CO2 laser cutting of Poly(methyl methacrylate) (PMMA) sheets. Influenza A virus specific aptamers are immobilized onto the electrodes by UV cross-linking. Impedance based measurements at a single frequency, measured over time, are used to detect the virus in a buffer solution.
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Acknowledgments
This work was funded by the European Union under the 7th Framework Programme FP7 (GA nr. 6040699).
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Kirkegaard, J., Rozlosnik, N. (2017). Screen-Printed All-Polymer Aptasensor for Impedance Based Detection of Influenza A Virus. In: Prickril, B., Rasooly, A. (eds) Biosensors and Biodetection. Methods in Molecular Biology, vol 1572. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6911-1_5
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DOI: https://doi.org/10.1007/978-1-4939-6911-1_5
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