Abstract
Food allergy has become a major public health problem worldwide. In the past two decades, development in molecular biology and immunology has led to many new techniques that had improved traditional methods in the food allergy field. These methods greatly facilitate identification, characterization, and quantification of food allergen and are certainly leading to better diagnostics and therapeutics for food allergic diseases. Here we review methods commonly used for food allergens.
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References
Sicherer SH, Sampson HA (2014) Food allergy: epidemiology, pathogenesis, diagnosis, and treatment. J Allergy Clin Immunol 133(2):291–307
Hefle SL, Nordlee JA, Taylor SL (1996) Allergenic foods. Crit Rev Food Sci Nutr 36:S69–S89
Schmidt G, Gadermaier G, Pertl H et al (2008) Production of recombinant allergens in plants. Phytochem Rev 7(3):539–552
Westermeier R (2014) Looking at proteins from two dimensions: a review on five decades of 2D electrophoresis. Arch Physiol Biochem 120(5):168–172
Jayasena S, Smits M, Fiechter D et al Comparison of six commercial ELISA kits for their specificity and sensitivity in detecting different major peanut allergens. J Agric Food Chem 63(6):1849–1855
Di Girolamo F, Muraca M, Mazzina O et al (2015) Proteomic applications in food allergy: food allergenomics. Curr Opin Allergy Clin Immunol 15(3):259–266
Tanabe S (2007) Epitope peptides and immunotherapy. Curr Protein Pept Sci 8(1):109–118
Frank R (2002) The SPOT synthesis technique—synthetic peptide arrays on membrane supports—principles and applications. J Immunol Methods 267(1):13–26
Lin J, Bardina L, Shreffler WG et al (2009) Development of a novel peptide microarray for large-scale epitope mapping of food allergens. J Allergy Clin Immunol 124(2):315–322 322.e1–3
Sathe SK, Teuber SS, Roux KH (2005) Effects of food processing on the stability of food allergens. Biotechnol Adv 23(6):423–429
Riemer A, Scheiner O, Jensen-Jarolim E (2004) Allergen mimotopes. Methods 32(3):321–327
Ponomarenko J, Bui HH, Li W et al (2008) ElliPro: a new structure-based tool for the prediction of antibody epitopes. BMC Bioinformatics 9:514
Liang S, Zheng D, Standley DM et al (2010) EPSVR and EPMeta: prediction of antigenic epitopes using support vector regression and multiple server results. BMC Bioinformatics 11:381
Salvat R, Moise L, Bailey-Kellogg C et al (2014) A high throughput MHC II binding assay for quantitative analysis of peptide epitopes. J Vis Exp (85), e51308, doi:10.3791/51308
Lin J, Sampson HA (2009) The role of immunoglobulin E-binding epitopes in the characterization of food allergy. Curr Opin Allergy Clin Immunol 9(4):357–363
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Lin, J., Alcocer, M. (2017). Overview of the Commonly Used Methods for Food Allergens. In: Lin, J., Alcocer, M. (eds) Food Allergens. Methods in Molecular Biology, vol 1592. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6925-8_1
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DOI: https://doi.org/10.1007/978-1-4939-6925-8_1
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6923-4
Online ISBN: 978-1-4939-6925-8
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