Abstract
Peroxisomes are ubiquitous dynamic and multifunctional organelles that contribute to numerous anabolic and catabolic pathways, being essential for human health and development. Their best known functions include the oxidation of fatty acids and metabolism of hydrogen peroxide with catalase as a marker enzyme. Indeed, historically, it was the cytochemical staining of catalase in many different cells and tissues that revealed the ubiquitous presence of peroxisomes in almost all animal and plant cells. In this chapter, the method for cytochemical staining of catalase with the alkaline 3, 3′-diaminobenzidine (DAB) is described. Since aldehyde fixation is a prerequisite for staining of catalase with DAB, a method for perfusion fixation of rat liver with glutaraldehyde is presented prior to the cytochemical staining method and the subsequent tissue processing for light and electron microscopy.
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Acknowledgments
It is a pleasure to thank all my former associates and colleagues for many years of fruitful and stimulating discussions. For technical support I would like to thank Gabi Krämer and Inge Frommer and for logistics Annemarie Achten, companions of many years. My work in USA was supported by grants from NIH and in Germany from DFG. In addition, we were supported by research funds of Boehringer Mannheim (now Roche Scientific).
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Fahimi, H.D. (2017). Cytochemical Detection of Peroxisomes in Light and Electron Microscopy with 3,3′-diaminobenzidine. In: Schrader, M. (eds) Peroxisomes. Methods in Molecular Biology, vol 1595. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6937-1_10
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DOI: https://doi.org/10.1007/978-1-4939-6937-1_10
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