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A Primate Model for Viral Hemorrhagic Fever

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Hemorrhagic Fever Viruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1604))

Abstract

Lymphocytic choriomeningitis virus strain WE (LCMV-WE), a Risk Group 3 virus, causes a disease in rhesus monkeys that closely resembles human infection with Lassa fever virus, a Risk Group 4 agent. Three stages of disease progression have been defined and profiled in this model: pre-viremic, viremic, and terminal. The earliest or pre-viremic stage reveals changes in the blood profile predictive of the later stages of disease. In order to identify whether specific changes are pathognomonic, it was necessary to perform a parallel infection with an attenuated virus (LCMV-Armstrong). Here we review the use of nonhuman primates to model viral hemorrhagic fever and offer a step-by-step guide to using a rhesus macaque model for Lassa fever.

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Authors and Affiliations

  1. University of Maryland School of Medicine, 725 W. Lombard Street, Baltimore, MD, 21201, USA

    Maria S. Salvato, Sandra Medina-Moreno & Joseph Bryant

  2. University of Louisville School of Medicine, Louisville, KY, 40292, USA

    Igor S. Lukashevich

  3. Institute of Infectious Diseases, First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang Province, People’s Republic of China

    Yida Yang

  4. Institute of Human Virology, University of Maryland School of Medicine, 725 W. Lombard Street, Baltimore, MD, 21201, USA

    Mahmoud Djavani & Juan Carlos Zapata

  5. Linea de zoonosis Emergentes y Re-emergentes, Grupo Centauro, Facultad de, Ciencias Agrarias, Universidad de Antioquia, Colombia

    Juan David Rodas

Authors
  1. Maria S. Salvato
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  2. Igor S. Lukashevich
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  3. Yida Yang
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  4. Sandra Medina-Moreno
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  5. Mahmoud Djavani
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  6. Joseph Bryant
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  7. Juan David Rodas
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  8. Juan Carlos Zapata
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Corresponding author

Correspondence to Maria S. Salvato .

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Editors and Affiliations

  1. School of Medicine, University of Maryland, Baltimore, Maryland, USA

    Maria S. Salvato

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Salvato, M.S. et al. (2018). A Primate Model for Viral Hemorrhagic Fever. In: Salvato, M. (eds) Hemorrhagic Fever Viruses. Methods in Molecular Biology, vol 1604. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6981-4_22

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  • DOI: https://doi.org/10.1007/978-1-4939-6981-4_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6980-7

  • Online ISBN: 978-1-4939-6981-4

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