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Incorporation of Artificial Lipid-Anchored Proteins into Cultured Mammalian Cells

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Lipidomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1609))

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Abstract

Exogenous lipid-anchored proteins can be incorporated into the plasma membranes of living mammalian cells, allowing the chemical structure of the incorporated protein and its lipid anchor to be controlled (and varied) to a much greater degree than is possible for proteins expressed by the cells themselves. This technology offers a variety of potential applications, including incorporating novel and complex protein constructs into cell surfaces and exploring structure-function relationships for biologically important lipid-anchored proteins such as glycosylphosphatidylinositol-anchored proteins. Here we describe detailed methods for stable incorporation of artificial lipid-anchored proteins into cultured mammalian cells under mild, nonperturbing conditions.

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Authors and Affiliations

  1. Department of Biochemistry, McGill University, Montréal, QC, Canada, H3G 1Y6

    Rania Leventis & John R. Silvius

  2. McIntyre Medical Sciences, Bldg. 3655, Promenade Sir-William-Osler Office, Room 815B, Montreal, QC, Canada, H3G 1Y6

    John R. Silvius

Authors
  1. Rania Leventis
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  2. John R. Silvius
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Corresponding author

Correspondence to John R. Silvius .

Editor information

Editors and Affiliations

  1. University of Miami, Miami, Florida, USA

    Sanjoy K. Bhattacharya

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Leventis, R., Silvius, J.R. (2017). Incorporation of Artificial Lipid-Anchored Proteins into Cultured Mammalian Cells. In: Bhattacharya, S. (eds) Lipidomics. Methods in Molecular Biology, vol 1609. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6996-8_20

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  • DOI: https://doi.org/10.1007/978-1-4939-6996-8_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6995-1

  • Online ISBN: 978-1-4939-6996-8

  • eBook Packages: Springer Protocols

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