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Defining Assembly Pathways by Fluorescence Microscopy

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Bacterial Protein Secretion Systems

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1615))

Abstract

Bacterial secretion systems are among the largest protein complexes in prokaryotes and display remarkably complex architectures. Their assembly often follows clearly defined pathways. Deciphering these pathways not only reveals how bacteria accomplish building these large functional complexes but can provide crucial information on the interactions and subcomplexes within secretion systems, their distribution within bacteria, and even functional insights. The emergence of fluorescent proteins has provided a new powerful tool for biological imaging, and the use of fluorescently labeled components presents an interesting method to accurately define the biogenesis of macromolecular complexes. Here we describe the use of this method to decipher the assembly pathway of bacterial secretion systems.

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Authors and Affiliations

  1. Laboratoire d’Ingénierie des Systèmes Macromoléculaires, Aix-Marseille Université, CNRS, UMR 7255, 31 Chemin Joseph Aiguier, 13402, Marseille Cedex 20, France

    Abdelrahim Zoued

  2. Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK

    Andreas Diepold

  3. Division of Infectious Diseases and Harvard Medical School, Department of Microbiology and Immunobiology, Howard Hughes Medical Institute, Brigham and Women’s Hospital, Boston, MA, USA

    Abdelrahim Zoued

  4. Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Str., 10, 35043 Marburg, Germany

    Andreas Diepold

Authors
  1. Abdelrahim Zoued
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  2. Andreas Diepold
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Correspondence to Andreas Diepold .

Editor information

Editors and Affiliations

  1. Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ - CNRS, Marseille, France

    Laure Journet

  2. Institut de Microbiologie de la Méditerranée, Aix-Marseille Univ - CNRS, Marseille, France

    Eric Cascales

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Zoued, A., Diepold, A. (2017). Defining Assembly Pathways by Fluorescence Microscopy. In: Journet, L., Cascales, E. (eds) Bacterial Protein Secretion Systems. Methods in Molecular Biology, vol 1615. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7033-9_23

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  • DOI: https://doi.org/10.1007/978-1-4939-7033-9_23

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7031-5

  • Online ISBN: 978-1-4939-7033-9

  • eBook Packages: Springer Protocols

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