Abstract
Somatic embryogenesis is considered the most effective method for commercial propagation of date palm. However, the limitation of obtaining synchronized development of somatic embryos remains an impediment. The synchronization of somatic embryo development is ideal for the applications to produce artificial seeds. Abscisic acid (ABA) is associated with stress response and influences in vitro growth and development. This chapter describes an effective method to achieve synchronized development of somatic embryos in date palm cell suspension culture. Among the ABA concentrations tested (0, 1, 10, 50, 100 μM), the best synchronized growth was obtained in response to 50–100 μM. Here we provide a comprehensive protocol for in vitro plant regeneration of date palm starting with shoot-tip explant, callus initiation and growth, cell suspension establishment, embryogenesis synchronization with ABA treatment, somatic embryo germination, and rooting as well as acclimatized plantlet establishment.
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References
Corredoira E, Vieitez AM, San José MC, Vieitez FJ, Ballester A (2016) Advances in somatic embryogenesis and genetic transformation of European chestnut: development of transgenic resistance to ink and blight disease. In: Park YS, Bonga JM, Moon HK (eds) Vegetative propagation of forest trees. National Institute of Forest Science (NIFoS), Seoul, Korea, pp 279–301
Ravi D, Anand P (2012) Production and applications of artificial seeds: a review. Int Res J Biol Sci 1:74–78
Colova VM, Bordallo PN, Phills BR, Bausher M (2015) Synchronized somatic embryo development in embryogenic suspensions of grapevine Muscadinia rotundifolia (Michx.) small. Vitis 46(1):15–18
Angoshtari R, Afshari RT, Kalantari S, Omidi M (2009) Effects of abscisic acid on somatic embryogenesis and induction of desiccation tolerance in Brassica napus. Asian J Plant Sci 8(4):276–284
Fernando SC, Weerakoon LK, Gunathilake TR (2004) Micropropagation of coconut through plumule culture. Cocos 16:1–10
Nambara E, Marion-Poll A (2005) Abscisic acid biosynthesis and catabolism. Annu Rev Plant Biol 56:165–185
Du L, Cai C, Wu S, Zhang F, Hou S, Gou W (2016) Evaluation and exploration of favorable qtl alleles for salt stress related traits in cotton cultivars (G. hirsutum L.) PLoS One 11(3):e0151076
Al-Khayri JM, Al-Bahrany AM (2004) Genotype-dependent in vitro response of date palm (Phoenix dactylifera L.) cultivars to silver nitrate. Sci Hortic 99:153–162
Al-Khayri JM, Al-Bahrany AM (2012) Effect of abscisic acid and polyethylene glycol on the synchronization of somatic embryo development in date palm (Phoenix dactylifera L.) Biotechnology 11(6):318–325
Galbiati M, Matus JT, Francia P, Rusconi F, Cañón P, Medina C et al (2011) The grapevine guard cell-related VvMYB60 transcription factor is involved in the regulation of stomatal activity and is differentially expressed in response to ABA and osmotic stress. BMC Plant Biol 11:142
Mishra P, Bhoomika K, Dubey RS (2013) Differential responses of antioxidative defense system to prolonged salinity stress in salt-tolerant and salt-sensitive Indica rice (Oryza sativa L.) seedlings. Protoplasma 250:3–19
Zouine J, El Bellaj M, Meddich A, Verdeil JL, El Hadrami I (2005) Proliferation and germination of somatic embryos from embryogenic suspension cultures in Phoenix dactylifera. Plant Cell Tissue Organ Cult 82(1):83–92
Sghaier-Hammami B, Jorrin-Novo JV, Gargouri-Bouzid R, Drira N (2010) Abscisic acid and sucrose increase the protein content in date palm somatic embryos, causing changes in 2-DE profile. Phytochemistry 71:1223–1236
Al-Khayri JM (2002) Growth, proline accumulation, and ion content in sodium chloride-stressed callus of date palm. In Vitro Cell Dev Biol Plant 38:79–82
Hassan MM, Gadalla EG, Abd-El Kareim AH (2008) Effect of sucrose and abscisic acid on in vitro growth and development of date palm during rooting stage. Arab J Biotech 11:281–292
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Al-Khayri JM (2013) Factors affecting somatic embryogenesis in date palm (Phoenix dactylifera L.) In: Aslam J, Srivastava PS, Sharma MP (eds) Somatic embryogenesis and genetic transformation in plants. Narosa Publishing House, New Delhi, pp 15–38
Al-Khayri JM (2005) Date palm Phoenix dactylifera L. In: Jain SM, Gupta PK (eds) Protocol for somatic embryogenesis in woody plants. Springer, Netherlands, pp 309–319
Acknowledgment
The authors wish to express gratitude to the Deanship of Scientific Research at King Faisal University, Saudi Arabia, for the financial support of this research project (Grant No. 120070).
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Alwael, H.A., Naik, P.M., Al-Khayri, J.M. (2017). Synchronization of Somatic Embryogenesis in Date Palm Suspension Culture Using Abscisic Acid. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Date Palm Biotechnology Protocols Volume I. Methods in Molecular Biology, vol 1637. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7156-5_18
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DOI: https://doi.org/10.1007/978-1-4939-7156-5_18
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7156-5
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