Abstract
The phytosterol-biotransforming strains were selected from Mycobacterium sp., using a high concentration of β-sitosterol. The selection was made by culturing the strains in a medium enriched with 14 g β-sitosterol/L as the unique source of carbon. During 2 months, the bacterial cultures were transferred successively. The extraction of the biotransformation products was made with methanol and ethyl acetate. The qualitative and quantitative analysis was made by means of thin-layer chromatography, gas-liquid chromatography (GLC), and GLC-mass spectrometry. Under these conditions, it was observed that after seven transfers, the strains Mycobacterium sp. MB-3683 and the Mycobacterium fortuitum B-11045 increased their biotransformation capacity from 20% to 64% and from 34% to 55%, respectively. The products in the highest proportion identified for each trial were androstenedione and androstadienedione. The results suggest that the high substrate concentration could be a selective mechanism to obtain strains more efficient in the biotransformation of β-sitosterol into steroidal bases.
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Acknowledgment
This work was supported by grant FONDEF D-96 1111, FONDECYT 1120664, and CONICYT/FONDAP/15130015.
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Mondaca, MA., Vidal, M., Chamorro, S., Vidal, G. (2017). Selection of Biodegrading Phytosterol Strains. In: Barredo, JL., Herráiz, I. (eds) Microbial Steroids. Methods in Molecular Biology, vol 1645. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7183-1_9
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DOI: https://doi.org/10.1007/978-1-4939-7183-1_9
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