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Reverse Phase Protein Arrays and Drug Discovery

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Proteomics for Drug Discovery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1647))

Abstract

Reverse Phase Protein Arrays (RPPA) represent a sensitive antibody-based proteomic approach, which enables simultaneous quantification of the abundance of multiple proteins and posttranslational modifications across multiple samples. Here, we provide protocols for RPPA performed on two distinct protein-binding substrates associated with two most commonly used RPPA platform technologies. We compare and contrast the respective advantages and limitations of each platform within the context of drug discovery applications.

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Author information

Authors and Affiliations

  1. Cancer Research UK Edinburgh Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XR, UK

    Kenneth G. Macleod, Bryan Serrels & Neil O. Carragher

Authors
  1. Kenneth G. Macleod
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  2. Bryan Serrels
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  3. Neil O. Carragher
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Corresponding author

Correspondence to Neil O. Carragher .

Editor information

Editors and Affiliations

  1. Department of Biological Sciences, Virginia Tech, Blacksburg, Virginia, USA

    Iulia M. Lazar

  2. Department of Pharmaceutical Sciences, School of Pharmacy, Southern Illinois University Edwardsville, Edwardsville, Illinois, USA

    Maria Kontoyianni

  3. ImmunoGen, Waltham, Massachusetts, USA

    Alexandru C. Lazar

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© 2017 Springer Science+Business Media LLC

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Macleod, K.G., Serrels, B., Carragher, N.O. (2017). Reverse Phase Protein Arrays and Drug Discovery. In: Lazar, I., Kontoyianni, M., Lazar, A. (eds) Proteomics for Drug Discovery. Methods in Molecular Biology, vol 1647. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7201-2_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7201-2_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7200-5

  • Online ISBN: 978-1-4939-7201-2

  • eBook Packages: Springer Protocols

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