Abstract
Elucidating the spatial resolution of gene transcripts provides important insight into potential gene function. MicroRNAs are short, singled-stranded noncoding RNAs that control gene expression through base-pair complementarity with target mRNAs in the 3′ untranslated region (UTR) and inhibiting protein expression. However, given their small size of ~22- to 24-nt and low expression levels, standard in situ hybridization detection methods are not amendable for microRNA spatial resolution. Here, I describe a technique that employs RNAscope probe design and propriety amplification technology that provides simultaneous single molecule detection of individual microRNA and its target gene. This method allows for rapid and sensitive detection of noncoding RNA transcripts in frozen tissue sections.
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Acknowledgment
This work was supported by Institutional Development Awards (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant numbers P20-GM104318 and P20-GM103423, and an American Heart Association Scientist Development Grant (11SDG7210045) to V.P.Y.
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Yin, V.P. (2018). In Situ Detection of MicroRNA Expression with RNAscope Probes. In: Gaspar, I. (eds) RNA Detection. Methods in Molecular Biology, vol 1649. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7213-5_13
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DOI: https://doi.org/10.1007/978-1-4939-7213-5_13
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7212-8
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