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Synthetic Tumor-Specific Promoters for Transcriptional Regulation of Viral Replication

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Mammalian Synthetic Promoters

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1651))

Abstract

Here we describe a collection of methods that have been adapted to isolate and modify tumor-specific promoters (TSPs) to drive viral replication for cancer therapy and other uses. We will describe as examples the secreted protein acidic and rich in cysteine (SPARC ) and the protease-activated receptor-1 (PAR-1) promoter. We outline strategies to select appropriate TSPs using bioinformatics resources and the methods utilized in their subsequent cloning, assessment of transcriptional activity, and their use in conditionally replicative oncolytic adenoviruses.

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Authors and Affiliations

  1. Laboratory of Molecular and Cellular Therapy, Leloir Institue—Conicet, Av Patricias Argentinas, 435, Buenos Aires, Argentina

    Maria Veronica Lopez, Eduardo G. Cafferata, Diego L. Viale & Osvaldo L. Podhajcer

Authors
  1. Maria Veronica Lopez
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  2. Eduardo G. Cafferata
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  3. Diego L. Viale
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  4. Osvaldo L. Podhajcer
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Corresponding author

Correspondence to Maria Veronica Lopez .

Editor information

Editors and Affiliations

  1. Department of Biochemical Pharmacology, William Harvey Research Institute, Queen Mary University of London, London, UK

    David Gould

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Lopez, M.V., Cafferata, E.G., Viale, D.L., Podhajcer, O.L. (2017). Synthetic Tumor-Specific Promoters for Transcriptional Regulation of Viral Replication. In: Gould, D. (eds) Mammalian Synthetic Promoters. Methods in Molecular Biology, vol 1651. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7223-4_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7223-4_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-7221-0

  • Online ISBN: 978-1-4939-7223-4

  • eBook Packages: Springer Protocols

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