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Northern Blot Detection of Virus-Derived Small Interfering RNAs in Caenorhabditis elegans Using Nonradioactive Oligo Probes

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Innate Antiviral Immunity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1656))

Abstract

Northern blot analysis has been widely used as a tool for detection and characterization of specific RNA molecules. When coupled with radioactive probe northern blot allows for robust detection and characterization of small RNA molecules of trace amount. Here, we describe the detection and size characterization of virus-derived small interfering RNAs (vsiRNAs) in C. elegans using nonradioactive DNA oligo probes in northern blotting. Our protocol allows for the detection and characterization of not only primary vsiRNAs but also secondary vsiRNAs, a class of single-stranded vsiRNAs that has distinct migration pattern, and can be easily adapted to the detection of vsiRNAs in other organisms.

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Acknowledgment

This work was supported by NIH grant R01GM119012-01A1 to R.L.

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Authors and Affiliations

  1. Department of Biological Sciences, Louisiana State University, 202 Life Science Building, Baton Rouge, LA, 70803, USA

    Tianyun Long & Rui Lu

Authors
  1. Tianyun Long
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  2. Rui Lu
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Corresponding author

Correspondence to Rui Lu .

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Editors and Affiliations

  1. Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada

    Karen Mossman

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Long, T., Lu, R. (2017). Northern Blot Detection of Virus-Derived Small Interfering RNAs in Caenorhabditis elegans Using Nonradioactive Oligo Probes. In: Mossman, K. (eds) Innate Antiviral Immunity. Methods in Molecular Biology, vol 1656. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7237-1_3

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  • DOI: https://doi.org/10.1007/978-1-4939-7237-1_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7236-4

  • Online ISBN: 978-1-4939-7237-1

  • eBook Packages: Springer Protocols

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