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Analysis of Exocyst-Positive Organelle (EXPO)-Mediated Unconventional Protein Secretion (UPS) in Plant Cells

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Plant Protein Secretion

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1662))

Abstract

Unconventional protein secretion (UPS) together with conventional protein secretion (CPS) is responsible for protein secretion in plants. We have previously identified a novel UPS pathway in plants, which is mediated by exocyst-positive organelle—EXPO. Here, we describe detailed protocols to study UPS in plants by using Arabidopsis protoplasts or transgenic suspension cells, expressing the EXPO marker Exo70E2-XFP, as materials. Via drug and osmotic treatment plus secretion assay, we illustrate several major methods to analyze EXPO-mediated UPS in plant cells, which also supplys mining tools for similar study.

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Acknowledgments

We are grateful to the wonderful discussion and suggestions from Prof. Liwen Jiang, the Chinese University of Hong Kong. This work was supported by grants from the 1000-Youth Elite Program of China, State Key Laboratory of Applied Microbiology Southern China (Grant No. SKYAM006-2016), and Science and Technology Program of Guangzhou, China (201604016068) to Y.D., and State Key Laboratory of Applied Microbiology Southern China (Grant No. SKYAM004-2016) to J.W.

There is no conflict of interest.

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Authors and Affiliations

  1. Department of Food Science & Engineering, College of Science and Engineering, Jinan University, Guangzhou, 510632, China

    Yu Ding

  2. College of Food Science, South China Agricultural University, Guangzhou, 510642, China

    Juan Wang

Authors
  1. Yu Ding
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  2. Juan Wang
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Corresponding authors

Correspondence to Yu Ding or Juan Wang .

Editor information

Editors and Affiliations

  1. School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, China

    Liwen Jiang

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Ding, Y., Wang, J. (2017). Analysis of Exocyst-Positive Organelle (EXPO)-Mediated Unconventional Protein Secretion (UPS) in Plant Cells. In: Jiang, L. (eds) Plant Protein Secretion. Methods in Molecular Biology, vol 1662. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7262-3_21

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  • DOI: https://doi.org/10.1007/978-1-4939-7262-3_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7261-6

  • Online ISBN: 978-1-4939-7262-3

  • eBook Packages: Springer Protocols

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