Abstract
Visualization of the intact embryo sac within the ovular/gynoecial tissues and clear identification of cell types can be logistically difficult and subject to interpretation. Cellular marker technologies have been available for the embryo sac, but have typically labeled only one cell type in a particular line. Here, we describe techniques for simultaneous labeling each cell type in the embryo sac and visualization methods for such in Arabidopsis, soybean, maize, and sorghum.
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Acknowledgment
This work was developed at and supported by DuPont Pioneer. Sorghum analysis was supported by a sub-award from the CSIRO under the Capturing Heterosis for Smallholder Farmers grant from the Bill and Melinda Gates Foundation. We thank Katherine Thilges, Tim Fox, Brian Loveland, Eric S. Caswell, April Agee, Ping Che, and others for technical support of this work. We thank Kristin Haug-Collet, Susan D. Johnson, and Anna M. Koltunow for critical reading of this manuscript.
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Chamberlin, M.A., Lawit, S.J. (2017). Development and Observation of Mature Megagametophyte Cell-Specific Fluorescent Markers. In: Schmidt, A. (eds) Plant Germline Development. Methods in Molecular Biology, vol 1669. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7286-9_5
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DOI: https://doi.org/10.1007/978-1-4939-7286-9_5
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