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DNA Accessibility by MNase Digestions

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Chromatin Immunoprecipitation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1689))

Abstract

Micrococcal nuclease (MNase) digestion of chromatin cuts linker DNA between neighboring nucleosomes and in this way generates mononucleosomes. The protected fragments can then be analyzed by genome-wide sequencing techniques or by quantitative PCR to obtain information about the positions of nucleosomes in the chromatin. Nucleosomes are differentially sensitive to MNase digestion, which means that titrations of MNase should be performed to obtain a comprehensive map of the nucleosome positions of a chromatin region or genome.

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Author information

Authors and Affiliations

  1. Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Svante Arrhenius väg 20C, 106 91, Stockholm, Sweden

    Ann-Kristin Östlund Farrants

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  1. Ann-Kristin Östlund Farrants
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Correspondence to Ann-Kristin Östlund Farrants .

Editor information

Editors and Affiliations

  1. Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden

    Neus Visa

  2. Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden

    Antonio Jordán-Pla

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Farrants, AK.Ö. (2018). DNA Accessibility by MNase Digestions. In: Visa, N., Jordán-Pla, A. (eds) Chromatin Immunoprecipitation. Methods in Molecular Biology, vol 1689. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7380-4_7

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  • DOI: https://doi.org/10.1007/978-1-4939-7380-4_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7379-8

  • Online ISBN: 978-1-4939-7380-4

  • eBook Packages: Springer Protocols

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